Comparison of community fungal and bacterial/archaeal diversity levels

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Discussion 2 1/13/2014.
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Comparison of community fungal and bacterial/archaeal diversity levels. Comparison of community fungal and bacterial/archaeal diversity levels. Numbers of unique ITS2 (blue circles) or 16S rRNA gene (orange diamonds) OTUs in 148 Human Microbiome Project samples (from 100 volunteers) that had been rarefied to 1,000 reads for both regions were determined. Plotted data represent the mean number of OTUs detected at the indicated number of samples when sample order was randomized over 1,000 iterations. The error bars represent the 95% confidence intervals. The ITS2 data most closely fit the power function y = 17.34x0.68 (R2 = 0.998), whereas the 16S rRNA gene data most closely fit the logarithmic equation y = 79.03ln(x) −17.84 (R2 = 0.988). Thomas A. Auchtung et al. mSphere 2018; doi:10.1128/mSphere.00092-18