Alignment of the L. maculans isocitrate lyase gene (icl1) with homologues. Alignment of the L. maculans isocitrate lyase gene (icl1) with homologues. (A)

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Alignment of the L. maculans isocitrate lyase gene (icl1) with homologues. Alignment of the L. maculans isocitrate lyase gene (icl1) with homologues. (A) Structure of a 5.8-kb region of L. maculans DNA including icl1 (exons are black) and an open reading frame with high sequence similarity to ribose-phosphate pyrophosphokinase (prs5) (exons are grey). Arrows under the genes indicate directions of transcription. The region deleted in the mutant (Δicl1) is indicated. (B) Alignment of L. maculans icl1 (L.ma) with isocitrate lyase genes from selected organisms: the ascomycetes Penicillium marneffei (P.ma; accession no. AF373018) and C. albicans (C.al; accession no. AF222905), the basidiomycete C. neoformans (C.ne; accession no. AF455253), the plant B. napus (B.na; accession no. L08482), and the bacterium M. tuberculosis (M.tu; accession no. AE007051). Alexander Idnurm, and Barbara J. Howlett Eukaryotic Cell 2002; doi:10.1128/EC.1.5.719-724.2002