Aim: How do certain factors, such as pH, temperature & enzyme shape affect enzyme action? Q: Why would someone want to take these supplements?
Enzymes All enzymes work best in optimal conditions
pH The peak (top) of the line graph shows the best pH for this enzyme
Effect of pH on 2 Different Enzymes
Questions Q1. In what kind of pH environment does enzyme pepsin function best? Q2. In what kind of pH environment does enzyme salivary amylase function best? Q3. What happens to the rate of enzyme activity when the pH is higher or lower than the optimal pH for a specific enzyme?
Temperature
Denaturation of Enzymes
Real World Denaturation
Summary High temperature and strong acids and bases can change the enzyme’s shape Result: Enzyme is denatured & cannot work The rate of enzyme activity decreases above and below optimal (best) enzyme conditions
Video https://app.discoveryeducation.com/learn/videos/5D232252-F66F-4083-A031-A5E7A992C97F
Exit Slip Answer the following questions on a piece of paper and turn answers in, before you leave class! Q1. List two factors that affect enzyme action. Q2. Explain denaturation. Q3. What site does a substrate molecule bind to, on an enzyme?