Specificity of the MeVA RT-qPCR assay, using an Applied Biosystems 7500 platform. Specificity of the MeVA RT-qPCR assay, using an Applied Biosystems 7500.

Slides:

Advertisements

Similar presentations

Results Alien Reference RNA QRT-PCR Detection Kit for Monitoring the Overall Performance of QRT-PCR Assays Bahram Arezi, Melissa McCarthy & Holly Hogrefe.

Advertisements

Commercial Assays and Detection of Parvovirus B19 genotypes Dr Sally A. Baylis, Division of Virology NIBSC.

Welcome to the presentation on the Welcome to the presentation on the

Good qPCR The Necessary and the Reasonable

Experience in Testing of Genotypes of B19

Vaccine specific RT-PCR for measles (MeVA)

Reverse Complement PCR: fast, low cost amplicon based NGS

Evidence for the involvement of a hematopoietic progenitor cell in systemic mastocytosis from single-cell analysis of mutations in the c-kit gene by A.

Summary of genetic alterations in resistant biopsies among patients progressing on ceritinib or alectinib. Summary of genetic alterations in resistant.

In Vivo Tropism of Hepatitis C Virus Genomic Sequences in Hematopoietic Cells: Influence of Viral Load, Viral Genotype, and Cell Phenotype by Hervé Lerat,

Human immunodeficiency virus facilitates infection/replication of hepatitis C virus in native human macrophages by Tomasz Laskus, Marek Radkowski, Joanna.

به نام خدایی که یاد او، مایه ی آرامش واقعی است.

ERT and uncoating assay shows increased vector capsid uncoating upon successful central DNA Flap formation. ERT and uncoating assay shows increased vector.

High levels of human herpesvirus 8 viral load, human interleukin-6, interleukin-10, and C reactive protein correlate with exacerbation of multicentric.

(A,B) mRNA in situ hybridisation (ISH) with Epstein–Barr virus-encoded small RNA-1 probe in a case of Hodgkin’s lymphoma focally affecting the bone marrow.

Distributions of parvovirus B19 genotype 1-3 in blood donations

Expression of retinitis pigmentosa protein RP1 in human bronchial epithelial cells. Expression of retinitis pigmentosa protein RP1 in human bronchial epithelial.

Comparison of a commercial and ‘in house’ assay for B19 DNA

De novo generation of infectious ZIKVNatal by CPER

Linearity of the RTx assay.

Comparisons of small-volume RTx assay results to bDNA and TaqMan results for clinical serum and plasma specimens for quantification of HCV RNA. (A) Linear.

Schematic representation of the HAV genome organization, translation products, and regions used for amplification. Schematic representation of the HAV.

Volume 116, Issue 3, Pages (March 1999)

Gel electrophoresis of measles virus cDNA amplicons (HU2 control measles virus RNA) amplified under optimised reaction conditions. Gel electrophoresis.

Wnt/β-catenin signaling regulates FGF signaling during fin regeneration. Wnt/β-catenin signaling regulates FGF signaling during fin regeneration. (A) fgf20a.

Identification of the major molecular types within C. gattii.

Transcriptional profiling of selected genes by RT-PCR.

Detection of Ebola virus infection in nonfatal versus fatal cases.

Fig. 3. The effect of VRC01 infusion on virus load in plasma and blood CD4 T cells during ART. The effect of VRC01 infusion on virus load in plasma and.

Increasing prevalence of multidrug resistance among P

RT-PCR analysis of LTBP expression in human cell lines.

Number of outbreaks associated with drinking water by water system type and year (n = 780), 1971 to “Other” includes outbreaks associated with bottled.

Transcription-mediated amplification (TMA).

MiRNA expression profiling in adipocytes from wild-type (WT) and leptin-deficient ob/ob mice. miRNA expression profiling in adipocytes from wild-type (WT)

Regional and cellular differences in Kcnq4 transcripts in the cochlea.

Target versatility. Target versatility. (A) Δctl0063, Δctl0064, Δctl0065, ΔtrpA, and wild-type C. trachomatis were analyzed by PCR with primers immediately.

Hector H. García et al. Clin. Microbiol. Rev. 2002; doi: /CMR

Scheme for recovery of viral nucleic acid from microarray spots.

Analysis of PHO1 Expression by RT-qPCR in the pru1 Mutant, Complementation Lines (COM5 and COM7), and Wild-type Plants during Pi Starvation. Analysis of.

Amplification curve from the MeVA RT-qPCR on the Roche LightCycler 480 system. Amplification curve from the MeVA RT-qPCR on the Roche LightCycler 480 system.

Reverse transcription (RT) in situ polymerase chain reaction (PCR) experiment using a single stranded biotinylated oligonucleotide probe for the detection.

Publications on biosensors for the field in general compared with the specific detection of whole bacteria. Publications on biosensors for the field in.

Multiplex PCR assay to detect common resistance determinants in B

Phylogenetic analyses showing the relationship between Ruminococcus, Blautia, and Clostridium on the basis of the sequences of the 16S region for each.

Recovery template. Recovery template. The recovery template (internal control) has the same sequence as the PCR product except the probe region has been.

Roberta B. Carey et al. Clin. Microbiol. Rev. 2018; doi: /CMR

Synchronization of a basic diagnostic algorithm (linked diagnostic tests) with different levels of diagnostic services. Synchronization of a basic diagnostic.

A, RT-PCR expression of matriptase-1 and matriptase-2 in a variety of 24 human cell lines. A, RT-PCR expression of matriptase-1 and matriptase-2 in a variety.

Discriminatory patterns of B. cenocepacia (A) and B

Genotypic differentiation (G-based) for all pairs of populations.

The Scorpions primer is an extension of molecular beacons.

Expression of S100A11 in bulk pancreatic tissues (pancreatic cancer, n = 22; IPMN, n = 18; nonneoplastic pancreas, n = 22). Expression of S100A11 in bulk.

Detection of PSA-mRNA by single (first panel, 710 bp) and nested (second panel, 455 bp) RT-PCR. Detection of PSA-mRNA by single (first panel, 710 bp) and.

Nested RT-PCR of Mammaglobin and CK19 mRNA in plasma and circulating cells of controls (C) and patients (T) with breast cancer. Nested RT-PCR of Mammaglobin.

Detection of mutant alleles using ddPCR.

Basic diagnostic algorithm to link the molecular line probe assay with solid culture- and liquid culture-based growth detection and susceptibility testing.

Significant differences in target copy numbers in multiplex PCRs may result in incorrect identification at high Cq values. Significant differences in target.

Reading and interpretation of mCIM results.

MTB1 to MTB3 Are Direct Transcriptional Targets of MYC2.

Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. PCR.

Rapid Detection of HIV-1 subtype C Integrase resistance mutations by the Use of High-Resolution Melting Analysis Tendai Washaya BSc, Msc. Pre-PhD Student.

MTB1 to MTB3 Negatively Regulate Diverse Aspects of JA Responses.

Relationship of partial rpoB gene sequences inferred by the neighbor-joining method for Canadian study strains of C. pyruviciproducens. Relationship of.

Negative effect of the use of the SuperScript III Platinum One-Step quantitative RT-PCR kit on the specificity of the MeVA RT-qPCR for the vaccine genotype.

Fig. 5 LASV replication in cynomolgus monkeys.

Dendrogram clustering the MALDI-TOF MSP obtained from at least 20 mass spectra of strains belonging to the C. haemulonii complex species and related species.

This figure shows the six phenotypes observed during CLI induction testing of S. aureus by disk diffusion. This figure shows the six phenotypes observed.

Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid. Confirmation of spliced RNA in cells transfected with a.

Detection of positive and negative KRBV genomic RNA strands as an indication of viral replication. Detection of positive and negative KRBV genomic RNA.

Primary GBM cells support productive HCMV replication in vitro.

Presentation transcript:

Specificity of the MeVA RT-qPCR assay, using an Applied Biosystems 7500 platform. Specificity of the MeVA RT-qPCR assay, using an Applied Biosystems 7500 platform. Synthetic RNA from the six active wild-type measles virus genotypes (B3, D4, D8, D9, G3, and H1) was tested. Panel A shows detection of 107 copies of RNA/reaction in the MeV qPCR assay, and panel B shows the lack of amplification of 107 copies of RNA/reaction in the MeVA RT-qPCR assay. The QuantiTect Probe RT-PCR kit was used for these reactions. Felicia Roy et al. J. Clin. Microbiol. 2017; doi:10.1128/JCM.01879-16