AAV8-mediated deletion of Tsc2 improves initial axon regeneration but not functional recovery after sciatic nerve crush injury. AAV8-mediated deletion.

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AAV8-mediated deletion of Tsc2 improves initial axon regeneration but not functional recovery after sciatic nerve crush injury. AAV8-mediated deletion of Tsc2 improves initial axon regeneration but not functional recovery after sciatic nerve crush injury. A, Injured sciatic nerve stained with SCG10 to label regenerating axons 3 d after crush. Larger images of yellow boxed region shown below whole nerve images. Red dotted line denotes the crush site while red arrowheads point to the three longest axons. Scale bars: 500 μm (top) and 50 μm (bottom). B, C, Quantification of average of the ten longest axons 3 d following sciatic nerve crush from (A) with linear regression (C) showing a lack of correlation between infection efficiency and length of longest axons. D, Paw withdrawal threshold measurements using von Frey test for recovery of mechanical sensitivity in injured foot pad at noted times after sciatic nerve crush. E, Time spent in pain behavior using acetone test for recovery from cold allodynia in uninjured and injured foot pad at noted times after sciatic nerve crush; *p < 0.05. BL, baseline; D, post-operative day. Extended Data Figure 9-1 shows data values of mean and SEM, number of replicates, statistical tests, and values for all comparisons. Dan Carlin et al. eNeuro 2019;6:ENEURO.0168-19.2019 ©2019 by Society for Neuroscience