Schematic diagram of MAPKKK5 constructs

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The Arabidopsis CERK1‐associated kinase PBL27 connects chitin perception to MAPK activation Schematic diagram of MAPKKK5 constructs. N: N‐terminal domain, KD: kinase domain, C: C‐terminal domain. MAPKKK5‐C interacts with PBL27 in yeast two‐hybrid experiments. The growth of yeast colonies on plates (‐ULWH) lacking uracil (U), leucine (L), tryptophan (W), and histidine (H) with 2 mM 3‐aminotriazole (3‐AT) indicates a positive interaction. Chitin‐induced MAPK activation was analyzed by immunoblots with α‐pMAPK. Complementation of chitin‐induced MAPK activation in mapkkk5 mutants by expression of MAPKKK5‐FLAG. MAPK activation was analyzed by immunoblots with α‐pMAPK. Flg22‐induced MAPK activation was analyzed by immunoblots with α‐pMAPK. Data information: Experiments (B–E) were performed three times with similar results. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 35, Issue: 22, Pages: 2468-2483, First published: 27 September 2016, DOI: (10.15252/embj.201694248)