Effect of LY2228820 on phosphorylation of various MAP kinase substrates in HeLa cells in vitro. Effect of LY2228820 on phosphorylation of various MAP kinase.

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Effect of LY2228820 on phosphorylation of various MAP kinase substrates in HeLa cells in vitro. Effect of LY2228820 on phosphorylation of various MAP kinase substrates in HeLa cells in vitro. HeLa cells (ATCC) were pretreated with LY2228820 (10 μmol/L → 9.8 nmol/L, 1:2 serial dilutions) for 1 hour before stimulation with anisomycin (10 μg/mL) for 45 minutes. Cells were then lysed in a protein lysis buffer (containing protease and phosphatase inhibitors) and proteins were analyzed by Western blotting for p38, JNK, and p44/42 (ERK1/2) pathway activities. The first 2 lanes represent HeLa lysates with and without anisomycin treatment (in absence of p38 inhibitor) serve as controls. Robert M. Campbell et al. Mol Cancer Ther 2014;13:364-374 ©2014 by American Association for Cancer Research