Supplementary Fig. 1 bp E F G H I Hybrid 1 SP2/0 MW P3X (-)

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Polymerase Chain Reaction

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Supplementary Fig. 1 bp E F G H I Hybrid 1 SP2/0 MW P3X (-) 850 650 500 850 E F G 400 H I MW SP2/0 P3X Hybrid 1 (-) Supplementary Fig. 1. PCR amplification of antibody heavy chain variable region from hybridoma-1 cell line using alternate primers. Following total RNA isolation and cDNA synthesis, heavy chain VR region from hybridoma-1 cell line (Hybrid-1) was amplified by PCR using 5 alternate PCR primer sets (E-I) and each product resolved by electrophoresis on 1.2% agarose gel. SP2/0 and P3X = parental myeloma cell lines. MW = molecular weight. bp = base pairs. (-) no template control.