Crystal structure of the nuclear effector of Notch signaling, CSL, bound to DNA CSL–DNA interactions. (B–D) were created with Molscript and Povscript (Kraulis,

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Crystal structure of the nuclear effector of Notch signaling, CSL, bound to DNA CSL–DNA interactions. (B–D) were created with Molscript and Povscript (Kraulis, 1991; Fenn et al, 2003). (A) Schematic representation of all protein–DNA interactions in the CSL–DNA complex. Specific interactions with the DNA bases are shaded in gray and nonspecific interactions are clear boxes. Hydrogen‐bonding or salt‐bridge interactions are denoted as an arrow and Van der Waals interactions are depicted as closed circles. (B) Stereo view of the major‐groove protein–DNA interactions of Arg234 and Asn26 from CSL with Gua8 and Gua9. The DNA is colored atom‐specifically: C yellow, N blue, O red, and P gray. The protein is colored blue with interacting loops solid and other parts transparent. (C) Stereo view of the interaction of Lys368 with Gua10 and Thy7′. Coloring is as in (B). (D) Stereo view of the minor‐groove interactions of the side chain of Gln401 with Ade13′ and the backbone carbonyl of Ser400 with Gua6. Coloring is as in (B). IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 23, Issue: 17, Pages: 3441-3451, First published: 05 August 2004, DOI: (10.1038/sj.emboj.7600349)