CARTmeso cell induction of antitumor antibodies.

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CARTmeso cell induction of antitumor antibodies. CARTmeso cell induction of antitumor antibodies. Sera obtained from patients pre- and post-CARTmeso therapy was analyzed for the presence of antibodies reactive with mesothelin and with cell extracts from autologous and allogeneic tumor cell lines. A, sera from MPM patient 17510-105 obtained at day −3 and day +64 were analyzed by immunoblotting for reactivity against purified mesothelin protein (MESO), SV40 large T antigen protein (SV), and lysates obtained from seven mesothelioma cell lines (REN, 208, 213, 302, 307, M30, and M60). New bands at ∼70 kDa (red boxes) and ∼45 kDa (blue boxes) were seen after CARTmeso cell infusion. B, sera from PDA patient 21211-101 obtained at days −7, +57, and +92 were analyzed by immunoblotting for reactivity against mesothelin protein (MESO), SV40 large T antigen protein (SV), lysate from a PDA cell line (Panc1), and lysate from a tumor cell line derived from the patient's own ascites (21211 tumor). A strong antimesothelin band was noted in the day +57 sample (blue arrow), and new bands were seen after receiving CARTmeso cell infusion at ∼65 to 80 kDa (red boxes) for the autologous 21211 cell line and for the Panc1 cell lines (blue boxes). Gregory L. Beatty et al. Cancer Immunol Res 2014;2:112-120 ©2014 by American Association for Cancer Research