Validation of the screen hits.

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Validation of the screen hits. Validation of the screen hits. A and B, Kaplan–Meier graphs of RFS for 225 TNBC and 1,415 non-TNBC patients in A or 1,286 ESR+ and 354 ESR− breast cancer patients in B from the same patient cohort, stratified by high and low expression of BCL10 (left graph) or IGF1R (right graph). High BCL10 and IGF1R expression resulted in worse RFS in TNBC and ESR− patients. The P values after Benjamini–Hochberg correction from log-rank (Mantel–Cox) test and the HRs (higher expression compared with lower expression) are shown. C, Reduction in ESR stress–induced XBP1s expression after CRISPR/Cas9-mediated knockout of BCL10 or siRNA-mediated knockdown of IGF1R. MCF7 or MDA-MB-231 cells deficient in BCL10 expression or IGF1R expression were treated with thapsigargin (TG, 300 nmol/L) or bortezomib (BTZ, 100 nmol/L) for 14 hours, and Western blotting was performed. Quantification of XBP1s/actin ratio is shown below each blot. Zhifen Yang et al. Mol Cancer Res 2018;16:745-753 ©2018 by American Association for Cancer Research