Fish Health Fish Kills, Reproductive Issues Multiple bacterial pathogens, but no consistent findings Aeromonas hydrophila and other motile Aeromonads Aeromonas salmonicida Flavobacterium columnare Multiple, often heavy parasite infestations Leeches, trematodes, myxozoans, cestodes Opportunistic fungal infections Largemouth Bass Virus Impaired Ecosystem Immunosuppression

Indicators of Estrogenic Endocrine Disruption Site No. Males Intersex Prevalence Severity % males w Vtg September 2005 Monocacy – U 11 82% 1.2 45% Monocacy – D 7 100% 1.8 33% April 2013 Monocacy - D 1.5 May 2014 13 77% 1.0

Feminization of Fishes Exposure to Environmental Estrogens Intersex in normally gonochoristic fishes Immature oocytes within testes Suggested as a marker of endocrine disruption Used as an indicator of exposure to estrogenic compounds