PUMA suppresses the growth of colon cancer cells.

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PUMA suppresses the growth of colon cancer cells. PUMA suppresses the growth of colon cancer cells. A, comparison of β-catenin levels in different cell types. Cell extracts were prepared from colorectal cancer lines (SW480, HCT116, DLD-1, and HT-29). Twenty micrograms of protein from each sample were subjected to Western blot analysis using anti-β-catenin and anti-β-actin antibodies. B, 293 cells were transiently transfected with human cDNA of caspase-8, Bid, Bak, Bax, PUMA, PKGIβ, and pcDNA3. Forty-eight hours after transfection, the percentage of apoptotic (sub-G1) cells was determined by FACS analysis. *, P < 0.05, significantly different from control. **, P < 0.01, significantly different from control. C, SW480 cell lines were transfected with constructs encoding PUMA and PUMA-ΔBH3. Cells were harvested 24 h after transfection, and equal cell numbers were diluted in duplicates in 10-cm dishes and grown under selection in hygromycin B for 3 wks, then fixed and stained with Coomassie blue. Colony formation in representative dishes (1:10 and 1:25 dilution) of transfected SW480 cells is shown. D, the number of colonies formed with cells infected with PUMA and PUMA-ΔBH3 is shown. *, P < 0.05, significantly different from PUMA-ΔBH3. Hadas Dvory-Sobol et al. Mol Cancer Ther 2006;5:2861-2871 ©2006 by American Association for Cancer Research