Abnormal morphologies are prevented by overexpression of CaN inhibitory peptide AKAP79 in APP/PS1 mouse brain. Abnormal morphologies are prevented by overexpression.

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Abnormal morphologies are prevented by overexpression of CaN inhibitory peptide AKAP79 in APP/PS1 mouse brain. Abnormal morphologies are prevented by overexpression of CaN inhibitory peptide AKAP79 in APP/PS1 mouse brain. A1 , In vivo low-magnification image of GFP-expressing neurites and axons (green), blood vessels containing Texas Red, and amyloid deposition stained with methoxy-XO4 (blue). Arrows indicated Aβ deposits. A2 , A3 , High-magnification live images taken from ∼100 μm below the brain surface in the neocortex of adult APP/PS1 mice show dendritic spines and dystrophies in both vector- and AKAP79-expressing conditions. B–D , Quantification of spine density ( B ), dendritic dystrophies ( C ), and neurite curvature ( D ) near Aβ deposits in vector- or AKAP79-expressing APP/PS1 mouse brain shows that AKAP79 expression decreases dystrophy size ( B ; vector, 16.4 ± 6.1 μm2; AKAP79, 12.8 ± 3.6; p = 0.029; n = 45 from 4 animals), increases spine density ( C ; vector, 17.8 ± 3.0/100 μm; AKAP79, 28.3 ± 6.1; p < 0.001; n > 400 spines), and decreases abnormal neurite curvature near plaques ( D ) (n = 4 animals for each condition and total > 400 spines). E , Postmortem sections indicated axonal dystrophies with SMI312 staining (red) and plaques with thioflavin S (blue) shows that plaque-associated axonal dystrophies are reduced (quantified in F ; vector, 16.4 ± 6.1 μm2; AKAP79, 12.8 ± 3.6; p = 0.0291; n = 45 plaques from 4 animals) in areas injected with AKAP79. F , Quantification of numbers of axonal dystrophies per single Aβ deposits from postmortem sections. Values represent mean ± SD. *p < 0.05; **p < 0.01. Hai-Yan Wu et al. J. Neurosci. 2010;30:2636-2649 ©2010 by Society for Neuroscience