Reverse transcription (RT) in situ polymerase chain reaction (PCR) experiment using a single stranded biotinylated oligonucleotide probe for the detection of the measles virus Nucleocapsid (N) gene in infected Vero cells. Reverse transcription (RT) in situ polymerase chain reaction (PCR) experiment using a single stranded biotinylated oligonucleotide probe for the detection of the measles virus Nucleocapsid (N) gene in infected Vero cells. Optimal cytoplasmic staining is achieved in (A) (1 μg/ml), higher probe concentrations in panels (B) (1.5 μg/ml) and (C) (2 μg/ml) showed increasing non-specific nuclear staining. V Uhlmann et al. Mol Path 2002;55:84-90 ©2002 by BMJ Publishing Group Ltd and Association of Clinical Pathologists