CNOT6L couples the selective degradation of maternal transcripts to meiotic cell cycle progression in mouse oocyte Live‐cell imaging results showing in vitro.

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Presentation transcript:

CNOT6L couples the selective degradation of maternal transcripts to meiotic cell cycle progression in mouse oocyte Live‐cell imaging results showing in vitro meiotic division of WT and Cnot6l−/− oocytes. Abbreviations: PM, prometaphase; MI, metaphase I; AI, anaphase I; TI, anaphase I‐metaphase II transition; MII, metaphase II; PB1: polar body‐1. Hours after released from GV arrest are indicated. Scale bar, 20 μm. Data represent the mean and standard deviations of mCherry‐securin fluorescence intensity in WT and Cnot6l−/− oocytes at each time point. Values from individual oocytes are normalized relative to that at 0 h after released from GV arrest. The numbers of analyzed oocytes are indicated (n). SMC3 immunofluorescence showing cohesin on chromosomes of WT and Cnot6l−/− oocytes at 16 h after culture. Centromeres and DNA were labeled by CREST and DAPI, respectively. Scale bar, 5 μm. BUB3 immunofluorescence on chromosome spreads made from WT and Cnot6l−/− oocytes at 9.5 h after culture. More than 8 oocytes were observed for each genotype with similar results. Scale bar, 5 μm. PB1 emission rates in WT and Cnot6l−/− oocytes cultured with or without MPS1 inhibitor reversin (5 μm). Reversin is added at 9 h after culture. Error bars, SEM. The numbers of analyzed oocytes are indicated (n). IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 37, Issue: 24, First published: 26 November 2018, DOI: (10.15252/embj.201899333)