A549 cells internalize NHF-ATP and NHF-ATP colocalizes with HMWFD

Slides:

Advertisements

Similar presentations

IL-1β significantly augments the angiogenic activity of NSCLC by inducing the expression of angiogenic CXC chemokine genes. IL-1β significantly augments.

Advertisements

Proline supplementation during P5CS protein knockdown suppressed GCN2 activation. Proline supplementation during P5CS protein knockdown suppressed GCN2.

nab-Paclitaxel promotes elevated intratumoral gemcitabine levels.

IL32 prompts cell activation and cancer-related pathways.

Confocal fluorescence microscopical images of 3-nitrotyrosine-positive blood vessels from a patient with mitochondrial disease. Confocal fluorescence microscopical.

The selective PI3K inhibitor A66 suppresses PIP3 accumulation, AKT phosphorylation at Thr308, and YAP/TAZ–regulated gene expression in PDAC cells. The.

Overexpression of HGF decreases MET protein levels.

Inflammatory stimuli downregulate macropinocytosis of exosomes in microglia. Inflammatory stimuli downregulate macropinocytosis of exosomes in microglia.

LSD1, a histone demethylase, is required for the assembly of the Notch repressor complex. LSD1, a histone demethylase, is required for the assembly of.

Exosome-mediated inhibition of T cells is reversible.

Adipocytes sequester daunorubicin (DNR).

A, B, apoptosis analysis using Annexin V-FITC/PI was done on 5th, 7th, and 9th day after transfection either with si control or si AEBP1in U87MG and U138MG.

Curcumin-generated ROS activates Chk1 and Chk2 kinases.

BCX inhibits lamellipodia formation in lung cells.

Modulation of Hh signaling modifies doxorubicin incorporation in leukemia cells. Modulation of Hh signaling modifies doxorubicin incorporation in leukemia.

CLIC1 promotes tumor cell spreading through effects on MLCK

Gramicidin A (GA) decreases HIF protein expression in RCC cells.

Synergistic effect of CNP and doxorubicin (DOX) on thiol oxidation.

CDCP1 is required for invadopodia formation and ECM degradation by human breast cancer cells. CDCP1 is required for invadopodia formation and ECM degradation.

A, ATP production by PC3 prostate cancer cells after treatment with oligonucleotide/Lipofectin complexes. a, ATP production by PC3 prostate cancer cells.

Activity of SW480 qHTS runs and podophyllotoxin titration–response profiles of from library and control samples. Activity of SW480 qHTS runs and podophyllotoxin.

Silencing hOGG1 triggers caspase-3 and caspase-7 activation in response to H2O2 in GM00637 cells. Silencing hOGG1 triggers caspase-3 and caspase-7 activation.

CDCP1 colocalizes and interacts with MT1-MMP.

Effect of LY on phosphorylation of various MAP kinase substrates in HeLa cells in vitro. Effect of LY on phosphorylation of various MAP kinase.

In vitro invasion assays were done in the Chemicon invasion chamber containing extracellular matrix. In vitro invasion assays were done in the Chemicon.

BME treatment increases granzyme B expression in NK 3.3 cells.

Selectivity of SHAL binding to human B-cell lymphoma cell lines.

Distribution of γ-H2AX foci on metaphase spreads from BLM-proficient and BLM-deficient cells. Distribution of γ-H2AX foci on metaphase spreads from BLM-proficient.

A, GP and GR epithelial tumor cells are equally responsive to gefitinib. A, GP and GR epithelial tumor cells are equally responsive to gefitinib. Equal.

Geldanamycin-induced endocytosis and down-regulation of ErbB2 in cells expressing ErbB2 only. Geldanamycin-induced endocytosis and down-regulation of ErbB2.

Binding and internalization of bispecific immunotoxins.

DNA damage-induced RPA foci in asynchronous cells labeled with BUdR.

CDCP1 localizes to intracellular vesicles containing lipid rafts.

Binding and antiproliferative effects of ANG4043 and anti-HER2 mAbs on tumor cells. Binding and antiproliferative effects of ANG4043 and anti-HER2 mAbs.

CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial cells. CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial.

Confocal microscopy analysis of SK-BR-3 and HC cells treated with CF488A-labeled HER2-lytic hybrid peptide. Confocal microscopy analysis of SK-BR-3 and.

Comparison of the frequency of nucleotide variation in the mtDNA D-loop region between stomach and other tumor cell lines. Comparison of the frequency.

A, P causes G2-M arrest with apoptosis in asynchronous population of PC-3 when exposed to 1.5 and 5 μmol/L P for various time points. A, P

Induction of DNA strand breaks by artesunate.

Localization of oxidated thiols in A375 melanoma cells.

SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression. SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression.

WP1066 inhibits the progression of OCIM2 cells through the cell cycle.

6-TG dose and time relationships for DSB formation using PFGE, demonstrating that the formation of DSBs in both MMR+ M4 and MMR− V2 cells is dose dependent.

An image-based assay that identifies EDR within cells.

MAPK/ERK signaling regulates TLR4 gene expression in response to BRAFV600E. MAPK/ERK signaling regulates TLR4 gene expression in response to BRAFV600E.

Induction of apoptosis by statins in NB4 cells and NB4 variant cell lines. Induction of apoptosis by statins in NB4 cells and NB4 variant cell lines. A,

TBK1 signaling also promotes basal autophagy in 8988T cells.

The activity of MRP1-EYFP is analogous to wild-type MRP1.

Three different endocytoses contribute to ATP internalization.

VEGF165 stimulates migration of HMVECs

Adhesion of T-cell clones to autologous tumor cells under shear stress

The activation of PKC-δ and JNK1 is essential for doxorubicin-induced senescence. The activation of PKC-δ and JNK1 is essential for doxorubicin-induced.

Inhibitory effects of nobiletin and luteolin on TPA-induced O2− generation in differentiated HL-60 cells and their cellular uptake. Inhibitory effects.

PTEN-deficient prostate cancer cells exhibit constitutive macropinocytosis. PTEN-deficient prostate cancer cells exhibit constitutive macropinocytosis.

AKT activation in HCC2429 is SRC- but not Notch-dependent.

TGFβ1/Smad3 signaling suppresses IFNγ production via an E4BP4-dependent mechanism in NK-92 cells. TGFβ1/Smad3 signaling suppresses IFNγ production via.

CpG oligodeoxynucleotide (CpG ODN)–induced activation of different types of primary malignant B cells. CpG oligodeoxynucleotide (CpG ODN)–induced activation.

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

Establishment of HeLa/rtTAA/TRE-N1-IC cell line.

Association of TCTP with Pim-3 in human pancreatic cancer cell lines.

Vorozole treatment-associated changes in protein abundance of multiple antigens. Vorozole treatment-associated changes in protein abundance of multiple.

Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated breast cancer cells. Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated.

Increased invasion does not require direct contact between neutrophils and UMSCC47. Increased invasion does not require direct contact between neutrophils.

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

T3 or T4 induces MAPK activation in myeloma cells.

Identification and characterization of activated integrin pathway module by signal transduction representation (A) and unsupervised hierarchical clustering.

I3C reduces the level of Cdc25A protein in breast cancer cells.

Purinergic receptor signaling on intracellular ATP levels and extracellular ATP on glycolytic and OXPHOS rates. Purinergic receptor signaling on intracellular.

Determination of O2− production by H&E staining using confocal fluorescence microscope. Determination of O2− production by H&E staining using confocal.

E2F4 program is predictive of the efficacy of intravesical BCG immunotherapy in NMIBC. A, the survival curves of intravesical therapy-treated and untreated.

Presentation transcript:

A549 cells internalize NHF-ATP and NHF-ATP colocalizes with HMWFD A549 cells internalize NHF-ATP and NHF-ATP colocalizes with HMWFD. A, A549 cells were incubated with both 10 μmol/L NHF-ATP (green) and HMWFD (red) for 30 minutes and then examined with fluorescence microscopy and quantified by ImageJ. A549 cells internalize NHF-ATP and NHF-ATP colocalizes with HMWFD. A, A549 cells were incubated with both 10 μmol/L NHF-ATP (green) and HMWFD (red) for 30 minutes and then examined with fluorescence microscopy and quantified by ImageJ. Inlets show colocalization of the two molecules. Contour of each cell was drawn based on photos of the same but overexposed cells. B, intracellular ATP levels in A549 cells treated with or without extracellular ATP or IPA-3, a macropinocytosis inhibitor. ***, P < 0.001. Yanrong Qian et al. Mol Cancer Res 2016;14:1087-1096 ©2016 by American Association for Cancer Research