CDDO-Me induces apoptosis independent of Bcl-2 level as well as p53 status in human NSCLC cells. CDDO-Me induces apoptosis independent of Bcl-2 level as.

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CDDO-Me induces apoptosis independent of Bcl-2 level as well as p53 status in human NSCLC cells. CDDO-Me induces apoptosis independent of Bcl-2 level as well as p53 status in human NSCLC cells. A, effects of CDDO-Me on the levels of Bcl-2 family proteins. The same whole-cell protein lysates from H460 cells used in Fig. 4B and C were used in this study. B, comparison of basal Bcl-2 level, p53 status, and cell sensitivity to CDDO-Me among the NSCLC cells. Whole-cell protein lysates for detection of basal Bcl-2 expression level were prepared from different cell lines with similar density. IC50 was calculated based on cell survival results in Fig. 2A. p53 status of the NSCLC cell lines were reported previously (18, 31). W, wild-type; M, mutant; N, null. C, detection of endogenous and exogenous Bcl-2 expression in neo- and Bcl-2-transfected cells. End, endogenous; Exo, exogenous. D, overexpression of Bcl-2 in H460 cells failed to protect cells from apoptosis induced by CDDO-Me. The transfectants were seeded in 96-well plates and treated with the indicated concentrations of CDDO-Me for 2 days. Cell survival was evaluated by SRB assay described in “Materials and Methods.” Points, mean of four replicate determinations; bars, ± SD. Kevin B. Kim et al. Mol Cancer Ther 2002;1:177-184 ©2002 by American Association for Cancer Research