Multiplex PCR assay to detect common resistance determinants in B

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Multiplex PCR assay to detect common resistance determinants in B Multiplex PCR assay to detect common resistance determinants in B. fragilis. Multiplex PCR assay to detect common resistance determinants in B. fragilis. Amplification was done with a set of primers designed for detecting five resistance genes, including carbapenems (cfiA), cephalosporins (cepA), clindamycin (ermF), metronidazole (nimA-F), and tetracycline (tetQ), plus a set of primers for the B. fragilis 16S rRNA gene (positive control). Lane 0, negative control; lane M, DNA standards. Lanes 1 to 11 were either single or multiple B. fragilis clinical isolates with previously determined resistance determinants. The multiplex PCR assay was able to determine all resistance determinants present in either single- or multiple-strain samples. Itzhak Brook et al. Clin. Microbiol. Rev. 2013; doi:10.1128/CMR.00086-12