Ribozymes: The hammerhead swings into action

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Ribozymes: The hammerhead swings into action Jennifer A Doudna  Current Biology  Volume 8, Issue 14, Pages R495-R497 (July 1998) DOI: 10.1016/S0960-9822(98)70316-1

Figure 1 Structure of the hammerhead ribozyme. (a) The secondary structure of the hammerhead shows stems I, II and III, the CUGA sequence of the catalytic pocket, and the scissile bond (red). (b) As revealed by X-ray crystallography, the hammerhead is shaped like a wishbone, with stems I and II emanating from the catalytic pocket and stem III at the base. Current Biology 1998 8, R495-R497DOI: (10.1016/S0960-9822(98)70316-1)

Figure 2 The cleavage-site phosphate conformational switch. (a) The modification adjacent to the cleavage site that slows down the cleavage reaction. The methyl group (green), added adjacent to the 5′-oxygen leaving group of the reaction, creates a kinetic bottleneck at the stage of scissile-bond cleavage. (b) The cleavage-site conformational switch. As shown on the left, the cleavage-site phosphate in the ground state of the hammerhead ribozyme is 90° away from the geometry required for an in-line attack mechanism. Any significant movement away from the canonical helical conformation would thus position it more favorably for in-line attack, as indicated at right. Current Biology 1998 8, R495-R497DOI: (10.1016/S0960-9822(98)70316-1)