CD94/NKG2A expression and presence is higher on tumor-infiltrating immune cells. CD94/NKG2A expression and presence is higher on tumor-infiltrating immune.

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CD94/NKG2A expression and presence is higher on tumor-infiltrating immune cells. CD94/NKG2A expression and presence is higher on tumor-infiltrating immune cells. A, Representative contour plots on CD94 and NKG2A staining on CD3+ T cells from paired tumor, paratumor, and PBMC samples from 1 patient with cancer (proportion of CD94/NKG2A+ T cells in tumor = 17.9%; in paratumor = 1.27%; and in PBMC = 0.3%). B, Correlative analysis on NKG2A and CD94 expression by MFI on tumor-derived CD3+ T cells. N, number of patients = 22 (esophageal cancer, n = 7; gastric cancer, n = 8; and colorectal cancer, n = 7); correlative analysis of nonparametric Spearman test, r2 = 0.9064; P < 0.001. C, The frequency of CD94/NKG2C+ T cells from paired tumor (TUMOR), paratumor (PARA), and PBMC samples. N, number of patients = 22 (esophageal cancer, n = 7; gastric cancer, n = 8; and colorectal cancer, n = 7). D, Correlative analysis between the frequency of CD94/NKG2A+ CD3+ TILs with the HLA-E expression by MFI on EpCAM-specific tumor cells (left) and the HLA-E expression by MFI on tumor-derived CD141+ cDC (right). N, number of patients = 10 (esophageal cancer, n = 3; gastric cancer, n = 4; and colorectal cancer, n = 3); correlative analysis of nonparametric Spearman test, r2 = 0.9241, P < 0.0001 (left); r2 = 0.8860, P < 0.0001 (right). E, The proportion of CD94/NKG2A+ populations of CD3+ T cells, CD8+ T cells, NKT cells, and NK cells from paired tumor, paratumor, and PBMC. N, number of patients = 22 (esophageal cancer, n = 7; gastric cancer, n = 8; and colorectal cancer, n = 7); one-way ANOVA with Tukey post hoc analysis. F, The proportion of CD94/NKG2A+ population of CD4+ T cells derived from paired tumor, paratumor, and PBMC. N, number of patients = 22 (esophageal cancer, n = 7; gastric cancer, n = 8; and colorectal cancer, n = 7). G, The proportion of CD94/NKG2A+ and CD94/NKG2A− populations of CD8+ TILs according to TNM stage of clinical prognosis. N, number of patients = 22; (esophageal carcinoma, n = 7; gastric carcinoma, n = 8; and colorectal carcinoma, n = 7); two-way ANOVA with Tukey post hoc analysis. H, The proportion of CD94/NKG2A+ CD8+ TILs (left), CD94/NKG2A− CD8+ TILs (middle), and total CD8+ TILs (right) according to maturation phenotype expression of CD27, CD45RA, and CCR7 in carcinoma. (N, number of patients = 22; esophageal carcinoma, n = 7; gastric carcinoma, n = 8; and colorectal carcinoma, n = 7); one-way ANOVA with Tukey post hoc analysis. Horizontal line, median; interval, 95% confidence; connecting lines, samples from the same patients; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant. Data represent with median ± SEM. Megat Abd Hamid et al. Cancer Immunol Res 2019;7:1293-1306 ©2019 by American Association for Cancer Research