TSA-mediated changes in cell cycle inhibitor proteins.

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TSA-mediated changes in cell cycle inhibitor proteins. TSA-mediated changes in cell cycle inhibitor proteins. A2780 control (left panels) and TSA-treated (right panels) cells were stained for the cell cycle-regulatory proteins p53, p21, and p27 as described in “Materials and Methods.” A representative photomicrograph of each antisera’s staining is presented, with the percentage of positively stained cells shown below each photomicrograph. The percentage of positively stained cells was determined by counting five separate areas of each slide using a Chromavision automated cell imaging system. Data are presented as means ± SD. Significance (P < 0.05) was determined by unpaired Student’s t test. The figure is representative of three independent experiments with similar results (original magnification, ×200). Kevin A. Strait et al. Mol Cancer Ther 2002;1:1181-1190 ©2002 by American Association for Cancer Research