Overexpression of miR-335, but not miR-29a, reduces in vitro carcinogenesis. Overexpression of miR-335, but not miR-29a, reduces in vitro carcinogenesis.

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Suppl. Figure 1 A B Suppl. Figure 1. Relative (A) miR-21 and (B) miR-224 expression levels in HCC tumors in different stages (I,II,III,IV) assessed by.

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Overexpression of miR-335, but not miR-29a, reduces in vitro carcinogenesis. Overexpression of miR-335, but not miR-29a, reduces in vitro carcinogenesis. A and B, stable transfection of SBC-5 cells to overexpress miR-335 or miR-29a was achieved using a lentiviral system. Screening of single colonies by qRT-PCR was used to select cell lines with the highest expression of miR-335 (SBC-5 miR-335+) or miR-29a (SBC-5 miR-29a+; n = 3/group) for subsequent studies. Stable SBC-5 cell line transfected with control lentivirus was used as control cell line (SBC-5 VectorCtrl). C and D, compared with SBC-5 VectorCtrl cells, cell migration and invasion were significantly reduced in SBC-5 miR-335+ cells, but not in SBC-5 miR-29a+ cells (n = 3/group, 2 replicate experiments). E, SBC-5 miR-335+ formed fewer colonies than SBC-5 VectorCtrl, whereas SBC-5 miR-29a+ was equivalent to SBC-5 VectorCtrl (n = 3/group, two replicate experiments). F, proliferation, assessed by the MTT assay, was lower in SBC-5 miR-335+ than that in SBC-5 VectorCtrl, whereas proliferation of SBC-5 miR-29a+ cells was equivalent or slightly increased compared with SBC-5 VectorCtrl cells (n = 5/group/time point, 3 replicate experiments; compared with control, *, P < 0.05; **, P < 0.01; ***, P < 0.001). Meng Gong et al. Mol Cancer Res 2014;12:101-110 ©2014 by American Association for Cancer Research