Antigen-specific CD8+ T cells express higher levels of PD-1 in animals that received the optimized SSX2 vaccine. Antigen-specific CD8+ T cells express.

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Antigen-specific CD8+ T cells express higher levels of PD-1 in animals that received the optimized SSX2 vaccine. Antigen-specific CD8+ T cells express higher levels of PD-1 in animals that received the optimized SSX2 vaccine. A, FFPE tumor sections obtained at necropsy were stained for CD8 and PD-1 expression. Shown are both the whole tumor sections (×10, assembled using Nikon Elements software) and higher powered images (×20) from representative animals from each treatment group. For the assembled tumor sections, the scale bar in the top left image indicates a length of 500 μm, and the red boxes indicate the regions where the higher-power images were taken. B and C, CD8+ T cells were isolated from splenocytes from tumor-bearing (B) or tumor-free (C) HHDII-DR1 mice that had received the indicated peptide or DNA vaccine treatment, and stained for SSX2 p41 or p103 with tetramers (separated in B, pooled in C) and for PD-1 expression. Shown are the mean fluorescence intensity (MFI) values for PD-1 expression on individual live/CD8+/tetramer+ events, with the median represented by the solid gray line. Each test represents splenocytes from at least 3 animals per group pooled together; *, P < 0.05 using a Mann–Whitney test. Brian T. Rekoske et al. Cancer Immunol Res 2015;3:946-955 ©2015 by American Association for Cancer Research