Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells. Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells.

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Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells. Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells. WM793 cells were transfected with control, Mcl-1, Bcl-2, or Bcl-XL siRNA as indicated. A. Seventy-two hours post-transfection, cell lysates were analyzed by Western blotting for Mcl-1, Bcl-2, or Bcl-XL. Total ERK1/2 was used as a loading control. B. Seventy-two hours post-transfection, WM793 cells were serum starved for 24 h and then replated onto agar-coated dishes in serum-free medium. After 48 h, cells were analyzed for cleaved caspase-3 using flow cytometry. X axis, fluorescence intensity; Y axis, cell counts, with percentages of cells staining positive in each condition. C. Quantitation of the data in B is presented as the average percentage of cells staining positive for cleaved caspase-3 from three independent experiments. Karen Boisvert-Adamo et al. Mol Cancer Res 2009;7:549-556 ©2009 by American Association for Cancer Research