MAPJD expression in lung cancers. MAPJD expression in lung cancers. A, expression of MAPJD gene in clinical samples of NSCLC, examined by semiquantitative RT-PCR. We prepared appropriate dilutions of each single-stranded cDNA prepared from mRNAs of clinical lung cancer samples, monitoring the level of β-actin (ACTB) expression as a quantitative control. B, expression of MAPJD gene in lung cancer cell lines. Top and bottom, derived from a single gel. C, immunohistochemical evaluation of representative samples from surgically resected adenocarcinoma (ADC) and squamous cell carcinoma (SCC) tissues, using anti-MAPJD polyclonal antibody. Chie Suzuki et al. Mol Cancer Ther 2007;6:542-551 ©2007 by American Association for Cancer Research