Relative Protein Quantification of CcmK4, RbcS, and CcmM in the Carboxysome under Different CO2 Levels and Light Intensities using Confocal Microscopy.

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Relative Protein Quantification of CcmK4, RbcS, and CcmM in the Carboxysome under Different CO2 Levels and Light Intensities using Confocal Microscopy. Relative Protein Quantification of CcmK4, RbcS, and CcmM in the Carboxysome under Different CO2 Levels and Light Intensities using Confocal Microscopy. (A) Confocal images of CcmK4-YFP, RbcS-YFP, and CcmM-YFP strains under Air/ML, CO2/ML, LL, and HL. Fluorescence foci (green) indicate carboxysomes, and cell borders were outlined by white dashed lines. Scale bar = 2 μm. (B) Violin plot of carboxysome intensities under Air/ML, CO2/ML, LL, and HL, normalized to kernel density ML peak values (peaks marked by white dashed lines). a.u., Arbitrary units. (C) Kernel density estimates of CcmL carboxysome copy number grown under Air/ML, CO2, LL, and HL detected by Slimfield and corrected for chlorophyll. Triple Gaussian fits are indicated as colored dashed lines with the summed fit in red. The percentage in each Gaussian is indicated aside. Yaqi Sun et al. Plant Cell 2019;31:1648-1664 ©2019 by American Society of Plant Biologists