Figure 1. (A) The PNIPAM-functionalized thermoresponsive substratum with nanopatterns. Capillary force lithography was used for the fabrication of the.

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Figure 1. (A) The PNIPAM-functionalized thermoresponsive substratum with nanopatterns. Capillary force lithography was used for the fabrication of the substratum, with subsequent modification using amine-terminated PNIPAM on the PET film. (B) Representative macroscopic image of a large-area, scalable substratum, and (C) an SEM image of the 800-nm nanopatterned surface. (D) A microscopic view of hPDLSCs cultured on the 800-nm width nanopatterned surface incubated at 37°C for 48 hours following cell seeding. The yellow arrow designates the direction in which the hPDLSCs were aligned (scale bar=200 μm). (E) Cell sheet detachment from the substratum after low-temperature treatment. An aligned and interconnected hPDLSC sheet with intact cell-to-cell junctions was slowly detached from the substratum as a single monolayer sheet upon water penetration from the periphery after being maintained at 22°C for 30 minutes. The white arrow indicates the direction of detachment (scale bar=200 μm). PNIPAM: poly(N-isopropyl-acrylamide), SEM: scanning electron microscopy, hPDLSC: human periodontal ligament stem cell, PUA: poly(urethane acrylate), GMA: glycidyl methacrylate, PET: polyethylene terephthalate, PGMA, poly(glycidyl methacrylate). Figure 1. (A) The PNIPAM-functionalized thermoresponsive substratum with nanopatterns. Capillary force lithography was used for the fabrication of the substratum, with subsequent modification using amine-terminated PNIPAM on the PET film. (B) Representative macroscopic image of a large-area, scalable substratum, and (C) an SEM image of… J Periodontal Implant Sci. 2017 Dec;47(6):402-415. https://doi.org/10.5051/jpis.2017.47.6.402