Fig. 2. Western blot analysis of purified and enriched OMVs derived from O. tsutsugamushi. (A) Blots were revealed with FS15 monoclonal antibody against.

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Fig. 2. Western blot analysis of purified and enriched OMVs derived from O. tsutsugamushi. (A) Blots were revealed with FS15 monoclonal antibody against OMVs and had similar patterns with blots of bacterial lysates. (B) Immunoblot of OMVs with polyclonal antibody showed a prominent 56-kDa protein band. (C) OMVs immunoenriched with FS15 monoclonal antibody revealed major surface antigens. ECV304, lysates of host cells; OT, lysates of purified O. tsutsugamushi; OMVs, outer membrane vesicles; FS15, mouse monoclonal antibody of O. tsutsugamushi; poly Ab, human polyclonal antibody purified from scrub typhus patient`s serum; IP, immunoprecipitation; IB, Immuno blot. Fig. 2. Western blot analysis of purified and enriched OMVs derived from O. tsutsugamushi. (A) Blots were revealed with FS15 monoclonal antibody against OMVs and had similar patterns with blots of bacterial lysates. (B) Immunoblot of OMVs with polyclonal antibody showed a prominent 56-kDa protein band. (C) OMVs immunoenriched with FS15 monoclonal antibody . . . J Korean Med Sci. 2015 Jul;30(7):866-870. http://dx.doi.org/10.3346/jkms.2015.30.7.866