Effects of somatostatin, somatostatin analogs, and endothelial cell somatostatin gene transfer on smooth muscle cell proliferation in vitro Rajabrata.

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Effects of somatostatin, somatostatin analogs, and endothelial cell somatostatin gene transfer on smooth muscle cell proliferation in vitro Rajabrata Sarkar, MD, PhD, Chris J. Dickinson, MD, James C. Stanley, MD Journal of Vascular Surgery Volume 29, Issue 4, Pages 685-693 (April 1999) DOI: 10.1016/S0741-5214(99)70315-0 Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig 1 Somatostatin production by β-galactosidase gene–transfected endothelial cells (BAG EC) and human somatostatin-transfected endothelial cells (HSS EC) in various serum concentrations. Transfected and selected endothelial cells were grown to confluence, and media were changed to indicated serum concentrations. After 24 hours, media were removed and assayed for somatostatin peptide. *P < .05 versus β-galactosidase gene media (n = 2 to n = 3). #P < .05 versus β-galactosidase gene media and versus human somatostatin 2.5% serum. Journal of Vascular Surgery 1999 29, 685-693DOI: (10.1016/S0741-5214(99)70315-0) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig 2 Effect of transfer of media conditioned by β-galactosidase gene–transfected endothelial cells (BAG EC) and human somatostatin-transfected endothelial cells (HSS EC) on growth of smooth muscle cells (SMCs). Media containing indicated concentrations of serum were conditioned with canine BAG ECs and HSS ECs every 24 hours and then were transferred to rat or canine SMCs daily. SMC cell number was determined after 72 hours (rat, n = 3; canine, n = 4). *P < .05 versus BAG media. Journal of Vascular Surgery 1999 29, 685-693DOI: (10.1016/S0741-5214(99)70315-0) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig 3 Canine smooth muscle cell (SMC) growth in presence of various endothelial cell (EC) types or somatostatin analog. Canine SMCs were grown in coculture for 96 hours with indicated types of canine ECs. Octreotide (10–10 mol/L) was added in indicated groups. No significant differences were noted among four groups that were cocultured with ECs (SMC + EC, SMC + β-galactosidase gene (BAG) EC, SMC + human somatostatin (HSS) EC, SMC + EC + octreotide). All groups, n = 4. *P < .05 versus SMC alone. Journal of Vascular Surgery 1999 29, 685-693DOI: (10.1016/S0741-5214(99)70315-0) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig 4 Effect of somatostatin (SS) analogs on DNA synthesis and cell proliferation of canine and rat smooth muscle cells (SMCs). Nitric oxide donor S-nitroso-N-acetylpenicillamine (SNAP; 0.1 mmol/L) was used as positive control for inhibition of proliferation. DNA synthesis (thymidine incorporation) was measured in serum-restimulated quiescent SMCs. Cell number was determined after 72 hours in presence of indicated concentrations of somatostatin analogs (n = 3 to n = 4). *P < .05 versus control. Journal of Vascular Surgery 1999 29, 685-693DOI: (10.1016/S0741-5214(99)70315-0) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig 5 Effect of angiopeptin (150 ng/mL, 75 μmol/L) on DNA synthesis in canine arterial explants (clear bars) and 72-hour cell proliferation of subcultured canine smooth muscle cells (SMCs; solid bars; n = 4). *P < .05 versus control. Journal of Vascular Surgery 1999 29, 685-693DOI: (10.1016/S0741-5214(99)70315-0) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions