Cloning, Gene Structure, and Expression Patterns of Nap1 and Pir1 Genes.(A) SSAP detection of LORE-1 element integrations in pir1-1 and nap1-1 mutant plants.

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Cloning, Gene Structure, and Expression Patterns of Nap1 and Pir1 Genes.(A) SSAP detection of LORE-1 element integrations in pir1-1 and nap1-1 mutant plants compared with parental lines L. japonicus Gifu and Miyakojima MG20. Cloning, Gene Structure, and Expression Patterns of Nap1 and Pir1 Genes.(A) SSAP detection of LORE-1 element integrations in pir1-1 and nap1-1 mutant plants compared with parental lines L. japonicus Gifu and Miyakojima MG20. Asterisk indicates new integration in the nap1-1 allele. Both bands represent the same integration event.(B)Pir1 gene structure. Positions of mutations in the pir1-1, pir1-2, pir1-3, pir1-4, and pir1-5 alleles are indicated.(C)Nap1 gene structure. Positions of the mutations in the nap1-1, nap1-2, and nap1-3 alleles are indicated. In (B) and (C), filled rectangles indicate exons, and thin lines indicate introns.(D) Relative levels of Nap1 and Pir1 mRNA in different wild-type lotus organs. The level in uninoculated roots is set to 1 for each gene. Bars represent 95% confidence intervals.(E) and (F) RT-PCR detection of 5′- and 3′-ends of the Nap1 transcript in leaves of the nap1-2 mutant and wild-type plants. In each panel: lanes 1 and 2 are wild-type and nap1-2 ubiquitin controls; lane 3 and 4 are wild-type and nap1-2 Nap1 transcripts; lane 5 is the size marker. Keisuke Yokota et al. Plant Cell 2009;21:267-284 ©2009 by American Society of Plant Biologists