Illustration of working hypothesis and biological impact of retained introns. Illustration of working hypothesis and biological impact of retained introns.

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Illustration of working hypothesis and biological impact of retained introns. Illustration of working hypothesis and biological impact of retained introns. We hypothesized that hypo-DNA methylation is correlated with intron retention events. Out-of-frame intron retention potentially causes a frame-shift of all of the downstream exons, which eventually produces mRNA that leads to a severe loss of function, such as a truncated protein or nonsense-mediated mRNA decay (NMD). Dongwook Kim et al. Mol Cancer Res 2018;16:461-469 ©2018 by American Association for Cancer Research