Dose-dependent repression of BORIS promoter activity by DNA methylation. Dose-dependent repression of BORIS promoter activity by DNA methylation. The BORIS.

Slides:

Advertisements

Similar presentations

Date of download: 9/19/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Germline Epigenetic Regulation of KILLIN in Cowden.

Advertisements

A B C D ** CpG-free Luc -7,200 -5,900 e2piNOS-luc -4,900 e1piNOS-luc

Relative luciferase activity Relative luciferase activity

The use of reporter genes to define DNA regulatory elements

Figure S3. Absence of DNA methylation in pre-cancerous thymocytes

A B C D Rao et al., 2012; Supplementary Figure S1 p = 0.03 p = NS

Fig. 1. Effects of MD-2 promoter SNPs on transcription activity

miR-133a positively regulated p53/p21 pathway.

Natalia Kholod, Jacques Boniver, Philippe Delvenne

Promoter-Specific Hypomethylation Is Associated with Overexpression of PLS3, GATA6, and TWIST1 in the Sezary Syndrome Henry K. Wong, Heather Gibson,

The C/EBPδ tumor suppressor is silenced by hypermethylation in acute myeloid leukemia by Shuchi Agrawal, Wolf-Karsten Hofmann, Nicola Tidow, Mathias Ehrich,

TCR‐mediated IL‐2 promoter activation in J.Vav1 cells.

Epigenetic mechanisms silence a disintegrin and metalloprotease 33 expression in bronchial epithelial cells Youwen Yang, PhD, Hans Michael Haitchi, MD,

Dual-Luciferase Activation Assay with Potential Targets of ZmbZIP22.

EP4 promoter activity is induced by sulindac sulfide in T98G cells.

Volume 134, Issue 5, Pages e3 (May 2008)

Demethylation of an NF-κB enhancer element orchestrates iNOS induction in osteoarthritis and is associated with altered chondrocyte cell cycle M.C. de.

JQ1 directly represses the promoter activities of BRCA1 and RAD51

Bortezomib sensitivity correlates with basal NF-κB activity in KP lung adenocarcinoma cell lines. Bortezomib sensitivity correlates with basal NF-κB activity.

Volume 124, Issue 3, Pages (March 2003)

C-MYC induces the transcription of LIG3 and PARP1 in FLT3/ITD- and BCR-ABL1–positive cells. c-MYC induces the transcription of LIG3 and PARP1 in FLT3/ITD-

ATF5 transactivates Egr-1 via the ATF5CON sites in the Egr-1 promoter.

Phospho-Twist1–induced TGF-β2 acts as an essential mediator of signaling cross-talk between TGF-βR/Smad2 and PI3K/Akt axes. Phospho-Twist1–induced TGF-β2.

The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis

Figure 2 Luciferase assays of transiently transfected HEK 293 cells with reporter constructs containing the 766-bp wild-type KCNJ18 or c.-542 T/A mutant.

Volume 16, Issue 8, Pages (August 2016)

Volume 60, Issue 3, Pages (September 2001)

Hypermethylation of the CpG Island Near the G4C2 Repeat in ALS with a C9orf72 Expansion Zhengrui Xi, Lorne Zinman, Danielle Moreno, Jennifer Schymick,

Keratinocyte growth factor promotes goblet cell differentiation through regulation of goblet cell silencer inhibitor Dai Iwakiri, Daniel K. Podolsky

MyoD Targets TAF3/TRF3 to Activate Myogenin Transcription

Supplementary Fig. S1 BCL2 5’flanking ctrl siRNA luc

Activation of PPARγ leads to inhibition of anchorage-independent growth of human colorectal cancer cells Jeffrey A. Brockman, Rajnish A. Gupta, Raymond.

Volume 21, Issue 6, Pages (December 2004)

TDP1 knockdown increases the sensitivity of rhabdomyosarcoma cell lines to CPT treatment. TDP1 knockdown increases the sensitivity of rhabdomyosarcoma.

MMAP is required for spindle dynamics regulation.

DNA methylation of the Xist gene.

Progestins activate vascular endothelial growth factor gene transcription in endometrial adenocarcinoma cells Michael D Mueller, M.D., Jean-Louis Vigne,

Volume 25, Issue 11, Pages e6 (December 2018)

Volume 21, Issue 6, Pages (December 2004)

Allopurinol up-regulates DR5 expression in PC-3 and DU145 cells.

Wild-type NSD3 is required for the blockade of differentiation in BRD4–NUT-expressing NMC cells. Wild-type NSD3 is required for the blockade of differentiation.

A and B, mRNA expression for ZNF423 (A) and BRCA1 (B) for lymphoblastoid cell lines with WT/WT (8 cell lines), WT/V (7 cell lines), and V/V (8 cell lines)

Figure 2. p62−/−MEF cells enhance the activation of NF-κB induced by TLR4. (A) Wild-type (WT) p62+/+MEF and p62−/−MEF cells were transfected with pBIIx-luc.

BRCA1 promoter methylation detected in the peripheral blood and corresponding tumor. BRCA1 promoter methylation detected in the peripheral blood and corresponding.

T-VISA is robust in ovarian cancer cell lines.

Protracted temozolomide (TMZ) treatment leads to acquired TMZ resistance in glioblastoma (GBM) cells in vivo. Protracted temozolomide (TMZ) treatment leads.

BORIS mRNA expression in DNMT deficient HCT116 cell lines.

CDDO-Me induces apoptosis independent of Bcl-2 level as well as p53 status in human NSCLC cells. CDDO-Me induces apoptosis independent of Bcl-2 level as.

SATB2-AS1 repressed Snail transcription depending on SATB2-mediated recruitment of HDAC1. SATB2-AS1 repressed Snail transcription depending on SATB2-mediated.

CTCF-Dependent Chromatin Insulator Is Linked to Epigenetic Remodeling

SiRNA silencing of CDH3, CLDN1, KRT23, and MMP7 (A–D) in adenoma and CRC cell lines significantly reduces cell proliferation. siRNA silencing of CDH3,

RhoH reconstitution in HCL represses CD11c promoter activity.

Effect of mutations of the SRE and RCE on the Akt activation of the Fra-1 promoter. Effect of mutations of the SRE and RCE on the Akt activation of the.

Effect of expressing an activated mutant (Rac V12) and an inactivated mutant (Rac N17) of Rac on PTHRP gene expression levels and on JNK activation. Effect.

Peptide dose-response curves were done to estimate the avidity of these HTL for peptide TARP1-14 (A) and TARP14-27 (B-E) using autologous PBMCs and dendritic.

Endogenously produced n-3 PUFAs inhibit endometrial cancer cell growth in vitro and in vivo. Endogenously produced n-3 PUFAs inhibit endometrial cancer.

FOXO-responsive 3X-IRS promoter activity is reduced in LNAI cells versus LNCaP cells. FOXO-responsive 3X-IRS promoter activity is reduced in LNAI cells.

A Splicing-Independent Function of SF2/ASF in MicroRNA Processing

Pirh2 represses p73-dependent transactivation.

Effect of siltuximab on paclitaxel sensitivity in ovarian cancer drug resistant cells. Effect of siltuximab on paclitaxel sensitivity in ovarian cancer.

PKD1 associates with β-catenin and negatively regulates β-catenin/TCF transcription activity. PKD1 associates with β-catenin and negatively regulates β-catenin/TCF.

RLM-RACE mapping the 5' end of BORIS mRNA.

Pdcd4 expression inhibits AP-1 transactivation.

VEGF165 stimulates migration of HMVECs

Comparison of Akt1 and Akt3 for their abilities to activate the Fra-1 promoter. Comparison of Akt1 and Akt3 for their abilities to activate the Fra-1 promoter.

Methylation targets of TET1 in vitro.

αvβ6 functional assays in the β cell line using blocking antibodies.

Fig. 7. JQ1 represses the enhancer-promoter interaction of BRCA1.

Volume 6, Issue 3, Pages (March 1997)

CREB1 binds at the proximal region of the TGFB2 promoter and induces its transcriptional activation. CREB1 binds at the proximal region of the TGFB2 promoter.

Presentation transcript:

Dose-dependent repression of BORIS promoter activity by DNA methylation. Dose-dependent repression of BORIS promoter activity by DNA methylation. The BORIS 5' promoter region insert was methylated in vitro with no enzyme (mock), HpaII and HhaI, or SssI methylases, then was re-ligated into pGL3-Basic and transfected into cell lines. (A) Confirmation of the methylation status of the BORIS promoter. Following in vitro methylation reactions, BORIS promoter fragments were digested with either HpaII (a methylation-sensitive restriction enzyme) or McrBC (a methylation-specific restriction enzyme). (B) Dual luciferase activity assay measurement of differentially methylated BORIS promoter constructs following transfection into 293, RKO, and HCT116 cell lines. The mean values from triplicate data points are plotted, along with error bars corresponding to 1 SD. Data from each cell line are normalized to the mock-methylated control of the same cell line. (C) Activity of BORIS promoter constructs following transfection into wild-type and DNMT1-/-, 3b-/- HCT116 cells. To illustrate differences in basal activity of the mock methylated constructs in the two cell lines, luciferase values were not normalized to mock-methylated control samples in this instance. Anna Woloszynska-Read et al. Cancer Immun 2007;7:21 Copyright © 2007 by Adam R. Karpf