Synchrotron X-ray imaging provides micron resolution within a neocortical volume. a, Microscopic visualization of cells, blood vessels, and dendrites within an X-ray–imaged volume of somatosensory cortex. Synchrotron X-ray imaging provides micron resolution within a neocortical volume. a, Microscopic visualization of cells, blood vessels, and dendrites within an X-ray–imaged volume of somatosensory cortex. Each panel shows one of three perspectives within the xyz coordinate framework (panels to the right are 11.5 µm wide, large panel to the left is 100 µm wide). b, Digital rendering of a manually reconstructed subset of blood vessels and cell bodies (nuclei highlighted) selected from within the neocortical volume. c, Photomicrographs of a subvolume within this sample, using µCT and EM to identify overlapping regions. These images were collected at three different pixel sizes (0.65 µm, 100 nm, 3 nm). In the left panel, a subset of a single virtual slice from an X-ray tomogram that spans the neocortical volume (0.65 µm pixel size). Outlined in blue to the right of this is a subset of the volume (within a) that highlights a configuration of three cell bodies and distinct proximal microvessels. This sample was subsequently serially sectioned and imaged in a scanning electron microscope. These cells are located in the EM dataset (inset), the ultrastructure of which is well preserved, even after µCT (right in red). Eva L. Dyer et al. eNeuro 2017;4:ENEURO.0195-17.2017 ©2017 by Society for Neuroscience