High and maximum containment laboratories

Outline Achieving Containment NIOH ASPIRE Facility NICD BSL4 Facility Containment Barriers HVAC systems and Control Architectural components Sterilisation systems NIOH ASPIRE Facility Background Safety and functional considerations Facility design NICD BSL4 Facility BSL4 laboratory work BSL4 facility design NICD Ebola Mobile Laboratory Facility location and assessment Laboratory setup

Achieving Containment Strategies Using: Inward directional airflow Isolation dampers Sealing service penetrations Backflow prevention Filtration Standard Operating Procedures

Containment Barriers Primary barriers (safety equipment) BSCs- all procedures Personal Protective Equipment (PPE) Isolators and Ventilated Racks Secondary barriers (facilities) Design parameters to protect people surrounding the laboratory and the community Separate building or isolated zone Special HVAC systems and controls Lab Ante Rm Sterilizer BSC Primary Containment Secondary Containment

Hvac systems and control Room exhaust is through double HEPA filtration (BIBO safe-change housings) Supply air is HEPA filtered Supply air terminals (room) are have HEPA filters for the unlikely event of the lab going positive Exhaust and supply air are interlocked, with fan redundancy System response and control managed via a BMS

Architectural components Stainless Steel Doors with Inflatable Door Seals Surface Finishes – Epoxy based (3mm Floor, Walls and Ceilings Painted with Water-based Epoxy)

Sterilisation systems Solid Waste Sterilisation – Autoclaves Effluent Waste Sterilisation

NIOH aspire facility: Background Investigation of airborne transmission of Mycobacterium tuberculosis (and other infectious agents within indoor environments); Ultraviolet Germicidal Irradiation fixtures are implemented to inactivate airborne pathogens such as M.tuberculosis; The objective to inactivate airborne infectious agents in the upper part of the room, while minimizing radiation exposure to persons in the lower part of the room; The efficacy of commercially available germicidal lamps in South Africa is not known; Testing will involve aerosolising M.tuberculosis in a room environment and to determine the ability of UVGI fixtures to inactivate M. tuberculosis in the test room;

aspire facility: Safety & functional considerations Exposure to M.tuberculosis (avirulent/attenuated); Environmental factors such as air velocity, air mixing, and relative humidity affect the efficacy of UVGI fixtures; Moveable ceiling; The aerosolised air sample collection and variable time intervals;

aspire facility: facility design Biosafety Level 3 (BSL3) design; Single-pass system with HEPA filtered exhaust; HEPA filtered supply air; Clean and dirty change rooms; Negative pressure cascade; Use of primary containment devices (Class II A2 BSC); Use of PAPRs;

aspire facility: facility design

aspire facility: facility design

aspire facility: facility design

Nicd bsl4 facility: laboratory work Diagnosis and Research Development, Production and Validation of Diagnostic Reagents and Assays Investigation of Emerging and Re-emerging Viral Diseases Training Developed Mobile Laboratory Unit (MLU) capacity

BSl4 facility: laboratory design

BSl4 facility: laboratory design

BSl4 facility: laboratory design

BSl4 facility: laboratory design

Nicd ebola mobile laboratory: sierra leone The NICD MLU was deployed, through the WHO-GOARN recruitment mechanism, in Sierra Leone, Freetown on 17 August 2014 as part of WHO EVD outbreak response in West Africa. Aug 24: Arrival of UN delegation, including Drs. Nabaro (UN) and Fukunaga (WHO) to visit the NICD Mobile Laboratory at Lakka Hospital, Freetown.   Aug 25: President of the Republic of Sierra Leone Dr Ernest Bai Koroma and other governmental officials delegation visiting the NICD MLU at Lakka Hospital. Aug 25: NICD MLU declared to be fully operational

mobile laboratory: Facility location & Assessment

mobile laboratory: Facility location & Assessment

mobile laboratory

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