SiRNA silencing of CDH3, CLDN1, KRT23, and MMP7 (A–D) in adenoma and CRC cell lines significantly reduces cell proliferation. siRNA silencing of CDH3,

Slides:

Advertisements

Similar presentations

Enhanced DNA damage in IDH1 mutant glioma cells.

Advertisements

Rab11-FIP3 silencing upregulates TCR-CD3 cell surface expression in an Lck-dependent manner. Rab11-FIP3 silencing upregulates TCR-CD3 cell surface expression.

KG-501 suppresses NSCLC/IL-1β CM–induced migration of HUVECs by regulating IL-1β–induced CXC chemokine gene expression in NSCLC cells. KG-501 suppresses.

CDK2 is highly expressed in colon cancer cells and curcumin selectively suppresses HCT116 cell proliferation. CDK2 is highly expressed in colon cancer.

IL-1β differentially regulates CXCL5 and CXCL8 protein secretion in NHTBE and NSCLC cells. IL-1β differentially regulates CXCL5 and CXCL8 protein secretion.

IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner. IL-1β stimulates CXCL5 and CXCL8.

miR-133a positively regulated p53/p21 pathway.

PMAIP1/NOXA gene amplification increases DLBCL vulnerability to BCL2 inhibitors. PMAIP1/NOXA gene amplification increases DLBCL vulnerability to BCL2 inhibitors.

Variability in protein concentration of urine over a 10-d collection from a single healthy volunteer. Variability in protein concentration of urine over.

Knockdown of DAO inhibits DNA damage–induced senescence.

Interfering with HMGA2 expression blocks transformation in metastatic lung cancer cells. Interfering with HMGA2 expression blocks transformation in metastatic.

TDP1 knockdown increases the sensitivity of rhabdomyosarcoma cell lines to CPT treatment. TDP1 knockdown increases the sensitivity of rhabdomyosarcoma.

Lorn Kategaya, Senthil K. Perumal, Jeffrey H. Hager, Lisa D. Belmont

Expression changes of specific epigenetic-controlled tumor-related genes by the prenatal/maternal BSp treatment. qRT-PCR and Western blot analysis were.

Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.

Allopurinol up-regulates DR5 expression in PC-3 and DU145 cells.

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. JAK3A572V mutation causes constitutive JAK3 activity.

Wild-type NSD3 is required for the blockade of differentiation in BRD4–NUT-expressing NMC cells. Wild-type NSD3 is required for the blockade of differentiation.

Gene expression of epigenetic modulators, HDAC1 and Dnmt1, and the histone acetylation modification patterns by the prenatal/maternal BSp dietary treatment.

Monitoring perturbation of ER-mitochondria interactions linked to genetic modulation of protein of the VDAC1/Grp75/IP3R1 complex by in situ PLA. Representative.

Inhibition of γ-secretase increased hair cell differentiation by upregulating Math1. a, Expression of Math1 in inner ear stem cells was increased after.

SiRNA directed at Ngn1 inhibited differentiation of inner ear stem cells to β-III tubulin-positive cells. a, Inner ear stem cells treated with siRNA to.

eNOS regulated IR-induced NO generation and EGFR signal activation.

TFAP2A interacts with HIF-1α and regulates VEGF expression and cell proliferation. TFAP2A interacts with HIF-1α and regulates VEGF expression and cell.

Cell viability assay and expression of COX protein.

Variation in dUTPase expression in cell lines.

Evaluation of top candidate clones for selective killing in bispecific antibody format. Evaluation of top candidate clones for selective killing in bispecific.

Silencing hOGG1 triggers caspase-3 and caspase-7 activation in response to H2O2 in GM00637 cells. Silencing hOGG1 triggers caspase-3 and caspase-7 activation.

The role of p53 in H2O2-mediated cell death of hOGG1-deficient H1299 lung carcinoma p53 null cells. The role of p53 in H2O2-mediated cell death of hOGG1-deficient.

Dietary plant extracts inhibit NF-κB activation in vitro.

Colony-forming capacity of CD34+ cells with and without CLL-1 expression. Colony-forming capacity of CD34+ cells with and without CLL-1 expression. CD34+

Overexpression of miR-335, but not miR-29a, reduces in vitro carcinogenesis. Overexpression of miR-335, but not miR-29a, reduces in vitro carcinogenesis.

PELP1 regulates the expression and activities of MMPs in ER-negative cells. PELP1 regulates the expression and activities of MMPs in ER-negative cells.

JMJD2B and CDK6 expressions are significantly correlated in various types of cell lines. JMJD2B and CDK6 expressions are significantly correlated in various.

Β-Cryptoxanthin at a concentration of 10 μmol/L decreases proliferation in HCT116 cells after 6 and 8 days of treatment. β-Cryptoxanthin at a concentration.

Dose-dependent repression of BORIS promoter activity by DNA methylation. Dose-dependent repression of BORIS promoter activity by DNA methylation. The BORIS.

Sensor siRNAs can be used in high-order combinations.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

MiR-200c is a PI3K–AKT signaling pathway regulator in CRC

Expression of CRC stem cell markers and L1 in CRC cells.

Peptide dose-response curves were done to estimate the avidity of these HTL for peptide TARP1-14 (A) and TARP14-27 (B-E) using autologous PBMCs and dendritic.

HLA-DR53–restricted HTL responses to peptide TARP1-14.

Immunohistochemistry for phosphorylated (S235) S6 ribosomal protein and quantitation of Ki67 labeling indices in vehicle- and WAY –treated Eker (Tsc2+/−)

Knockdown of PKM2 suppressed TPA-induced skin cell transformation.

TFAP2A knockdown inhibits cell growth by targeting HIF-1α signaling.

IGF-IR in human head and neck cancer.

The effect of different concentrations of WMC-79 and time of exposure on the growth of HCT-116 cells. The effect of different concentrations of WMC-79.

Effects of HDAC2 inactivation on the invasive potential of human gastric cancer cells. Effects of HDAC2 inactivation on the invasive potential of human.

The effects of HDAC2 knockdown on cell-cycle proteins.

Substitution of endogenous Reptin with D299N mutant leads to HuH7 cell growth arrest. Substitution of endogenous Reptin with D299N mutant leads to HuH7.

FBXL19-AS1 knockdown inhibits proliferation, migration and invasion, and reduces the expression levels of angiogenesis related proteins in lung cancer.

Osteoactivin expression is required for the invasive phenotype of in vivo selected bone metastatic 4T1 breast cancer cells. Osteoactivin expression is.

Progastrin staining in colon adenomas and adenocarcinomas.

VEGF165 stimulates migration of HMVECs

Effects of ASGR2 knockdown on the phenotypes of MKN1 cells.

Effect of bevacizumab on the proliferation of A2780 cells.

Levels and activity of c-myc are increased in a series of stably transfected DAOY and UW228 medulloblastoma cell lines. Levels and activity of c-myc are.

HOXA11-AS acts as a ceRNA for miR-1297.

Decreased dopamine uptake following downregulation of SLC6A3.

Validation of NAA's cancer specificity in lung cancer.

A, Proportion for different in silico predictions at the RNA level.

Transfection efficiency and PicoGreen cell survival assays.

PARP1 suppresses the transcription of PD-L1 in cancer cells.

CPGL is a growth suppressor in pancreatic cancer cell lines.

Wild-type and oncogenic Ras differentially regulate cell proliferation

Curcumin decreases viability and proliferation of Bcr-Abl-expressing cells. Curcumin decreases viability and proliferation of Bcr-Abl-expressing cells.

Effects of inhibitors of PI3K, MEK1, and GSK-3β on visfatin-induced proliferation in HepG2 cells. Effects of inhibitors of PI3K, MEK1, and GSK-3β on visfatin-induced.

Expression of the angiogenic phenotype in the 4-NQO mouse model of HNSCC. A, tissue sections containing areas of normal, hyperkeratosis, dysplasia, and.

Determination of Per2 (left), MTNR1A (middle), and ERβ (right) protein expression levels in mammary tissues of rats sacrificed at ZT12. Determination of.

Sp1 complementation assay.

Presentation transcript:

siRNA silencing of CDH3, CLDN1, KRT23, and MMP7 (A–D) in adenoma and CRC cell lines significantly reduces cell proliferation. siRNA silencing of CDH3, CLDN1, KRT23, and MMP7 (A–D) in adenoma and CRC cell lines significantly reduces cell proliferation. PCNA protein was quantitated in transfected CRC and adenoma cells. All assays are done in triplicate, cell lines are grouped by phenotype (MSI: HCT116, LoVo; CIN: SW48, RKO; CIMP: FET, SW480), and results are normalized to tubulin and PCNA expression in csiRNA. Error bars note SD, and calculated P-values are for differences in PCNA expression in csiRNA and each phenotype. csiRNA (control nontargeting siRNA), **P < 0.0001. Elizabeth K. Broussard et al. Cancer Prev Res 2013;6:666-674 ©2013 by American Association for Cancer Research