IDH3α interacts with cSHMT during S phase at the nuclear lamina

Slides:

Advertisements

Similar presentations

Fig. 5 Correlation of RNA expression and protein abundance.

Advertisements

Fig. 4 3D reconfiguration of liquid metals for electronics.

Fig. 5 MicroLED array with 3D liquid metal interconnects.

Fig. 3 Cytosolic delivery of fluorescent proteins.

Fig. 3 The electrical contact of direct-printed and reconfigured liquid metals. The electrical contact of direct-printed and reconfigured liquid metals.

Fig. 2 Boronic acid–rich dendrimer in cytosolic delivery.

Fig. 2 Global production, use, and fate of polymer resins, synthetic fibers, and additives (1950 to 2015; in million metric tons). Global production, use,

Fig. 3 Projected gains in MCP.

Fig. 1 Pump-probe signatures of vermilion (red HgS), black HgS, and metallic Hg. Pump-probe signatures of vermilion (red HgS), black HgS, and metallic.

Fig. 3 Oil, gas, and FP water variations with time.

Fig. 1 Map of water stress and shale plays.

Fig. 4 Super-resolution live imaging of calcium containing vesicle transports via lysosomes. Super-resolution live imaging of calcium containing vesicle.

Fig. 1 Examples of experimental stimuli and behavioral performance.

Fig. 2 Some examples of weekly forecasts (the number of the forecasts are reported on Table 1). Some examples of weekly forecasts (the number of the forecasts.

Fig. 4 Resynthesized complex boronic acid derivatives based on different scaffolds on a millimole scale and corresponding yields. Resynthesized complex.

Fig. 6 Comparison of properties of water models.

Fig. 2 Reference-fixing experiment, results.

Fig. 3 Scan rate effects on the layer edge current.

Fig. 1 Product lifetime distributions for the eight industrial use sectors plotted as log-normal probability distribution functions (PDF). Product lifetime.

Fig. 1 Reference-fixing experiment, setup.

Fig. 5 Structural photocycle for DmCry.

Fig. 1 Neu1−/− fibroblasts have characteristics of myofibroblasts.

Fig. 1 Bioinspired design of AAD for promoting wound contraction.

Fig. 1 Parameterization and temporal distribution of carbon isotopic events in the database. Parameterization and temporal distribution of carbon isotopic.

Fig. 3 Forward model. Forward model. Summary of the resampled Monte Carlo simulations shown as histograms for epoch 1 (red), epoch 2 (green), and epoch.

Fig. 3 Photon number statistics resulting from Fock state |l, S − l〉 interference. Photon number statistics resulting from Fock state |l, S − l〉 interference.

Fig. 2 2D QWs of different propagation lengths.

Fig. 6 Longevity and mechanical perturbation of a liquid membrane.

Fig. 6 WPS imaging of different chemical components in living cells.

Fig. 6 Caspase-3– and -7–deficient cells maintain mitochondrial membrane polarity following intrinsic or extrinsic apoptotic stimuli. Caspase-3– and -7–deficient.

Fig. 1 IDH3α expression is elevated in human-derived gliomas.

Fig. 1 Histograms of the number of first messages received by men and women in each of our four cities. Histograms of the number of first messages received.

Fig. 5 Schematic phase diagrams of Ising spin systems and Mott transition systems. Schematic phase diagrams of Ising spin systems and Mott transition systems.

Fig. 4 OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with different pH. OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with.

Fig. 2 Regulation of CD73 and CD39 cell membrane expressions and extracellular adenosine levels by ERs in osteoprogenitor cells. Regulation of CD73 and.

Fig. 1 Average contribution (million metric tons) of seafood-producing sectors, 2009–2014. Average contribution (million metric tons) of seafood-producing.

Fig. 3 Production of protein and Fe(II) at the end of growth correlated with increasing concentrations of ferrihydrite in the media that contained 0.2.

Fig. 5 The different fractions of the rates of energy flow in the oscillating thermal circuit for the experiment with L = 58.5 H shown in Fig. 4A. The.

Fig. 2 IRF8 is expressed in CD68+ macrophages after SCI.

Fig. 1 Deficient CD73 and CD39 expressions and extracellular adenosine concentration in BM of OVX animals. Deficient CD73 and CD39 expressions and extracellular.

Fig. 4 SPICE simulation of stochasticity.

Fig. 2 NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ clean air policy. NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ.

Fig. 1 Size fractions of MPPs in different fertilizers.

Blue particles observed embedded within archaeological dental calculus

Fig. 2 Sources of plastic waste imports into China in 2016 and cumulative plastic waste export tonnage (in million MT) in 1988–2016. Sources of plastic.

Fig. 4 Relationships between light and economic parameters.

Fig. 5 Comparison of the liquid products generated from photocatalytic CO2 reduction reactions (CO2RR) and CO reduction reactions (CORR) on two catalysts.

Fig. 1 Location of the Jirzankal Cemetery.

Fig. 4 CO2 emission changes triggered by the JJJ clean air policy.

Fig. 4 Next-generation MOF with increased productivity.

Fig. 6 MSC encapsulation in vitro within PdBT cross-linked gels.

Multiplexed four- and eight-channel devices for rapid processing

Fig. 2 RVFV causes pathology within the liver, uterus, and placenta of pregnant dams. RVFV causes pathology within the liver, uterus, and placenta of pregnant.

Fig. 4 Mapping of abundance of the most dominant bacterial and archaeal phyla across France. Mapping of abundance of the most dominant bacterial and archaeal.

Fig. 4 Spatial mapping of the distribution and intensity of industrial fishing catch. Spatial mapping of the distribution and intensity of industrial fishing.

Fig. 4 Single-particle contact angle measurements.

Fig. 2 Supraballs and films from binary SPs.

Fig. 3 Performance of the generative model G, with and without stack-augmented memory. Performance of the generative model G, with and without stack-augmented.

Fig. 5 Reconstitution of the dormant state using fetal ovaries.

Fig. 1 Schematic structure of a fluorescently labeled eGLP1-conjugated MALAT1 ASO and internalization of fluorescent eGLP1 and eGLP1-MALAT1-ASO. Schematic.

Fig. 2 Comparison between the different reflective metasurface proposals when θi = 0° and θr = 70°. Comparison between the different reflective metasurface.

Fig. 1 Design principle and SEM characterization of super-origami DNA nanostructures with n-tuples. Design principle and SEM characterization of super-origami.

Fig. 1 Overview of amber clast with synchrotron x-ray μCT image of articulated snake skeleton (DIP-S-0907). Overview of amber clast with synchrotron x-ray.

Fig. 1 Doping schematics and optical properties.

Fig. 4 Effects of individual picosecond and microsecond pulses.

Fig. 2 IDH3α regulates GBM progression in vivo.

Fig. 3 Three small molecules inhibit the transport of CCR5 and do not affect transport of CCR1 or CXCR4. Three small molecules inhibit the transport of.

Mitochondrial import of CerS1 from smooth ER mediates lethal mitophagy

Fig. 3 4n hybrids undergo bipolar and tripolar mitosis.

Fig. 2 Time series of secularization versus GDP per capita, from four illustrative countries, over the 20th century. Time series of secularization versus.

Presentation transcript:

Fig. 4 IDH3α interacts with cSHMT during S phase at the nuclear lamina. IDH3α interacts with cSHMT during S phase at the nuclear lamina. (A) Cell cycle distribution in unsynchronized and S phase–arrested NHA cells as indicated by propidium iodide staining and flow cytometry–based quantification of G1, G2-M, and S phase content. un, unsynchronized; S, synchronized. (B) WB analysis of IDH3α in heavy membrane (HM) and cytosolic (C) compared to nuclear (Nuc) fractions. Histone H3 and cytochrome c oxidase subunit IV (COXIV) are shown as nuclear and mitochondrial markers, respectively. (C) Confocal IF images of unsynchronized (a and b) and S phase–arrested NHAs (c) stained for IDH3α (green), cytochrome c (cyto c; red), and DNA (Hoechst; blue). Scale bars, 20 μm (a), 14 μm (b), and 22 μm (c). Arrows point to IDH3α colocalizing with cytochrome c, and arrowheads point to IDH3α associated with the nuclear lamina. (D) Venn diagram illustrating IDH3α co-immunoprecipitated proteins using IgG control or IDH3α-specific antibodies in shScr- and shIDH3α-expressing GICs. (E) Bar graph illustrating total normalized cSHMT spectra in IgG and IDH3α immunoprecipitates in the indicated cell lines. (F) IP-WB analysis of IDH3α and cSHMT validates the IDH3α-cSHMT complex. Jasmine L. May et al. Sci Adv 2019;5:eaat0456 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).