Pulse treatment of actinomycin D shows a biphasic effect on LTAM

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Pulse treatment of actinomycin D shows a biphasic effect on LTAM Pulse treatment of actinomycin D shows a biphasic effect on LTAM. a, Young adult worms were treated for 5 min with DMSO or actinomycin D in DMSO at different time points after conditioning, and LTAM was tested 24 h after conditioning. Pulse treatment of actinomycin D shows a biphasic effect on LTAM. a, Young adult worms were treated for 5 min with DMSO or actinomycin D in DMSO at different time points after conditioning, and LTAM was tested 24 h after conditioning. The chemotaxis index for each treatment was calculated as described previously (Bargmann et al., 1993). The graph represents mean and SE. n = 6–16. Statistical significance was assessed with one-way ANOVA and Dunnett's post hoc test. Treatment conditions were compared with the untreated worms' 24 h memory. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05. b, Effect of the actinomycin D on RNA transcription was tested by measuring the RNA levels of tba-1 transcripts at different time points after 5 min pulse treatment with actinomycin D. tba-1 mRNA levels were normalized against bacterial spike RNA added to the isolated total RNA before reverse transcription reaction. The graph represents mean and SE of three independent experiments with three technical replicates each. c, Enrichment of ubiquitous, neuronal, or muscle-specific genes after PAB-1–mRNA complex purification using tissue-specific expression of PAB-1. The specific gene abundance was normalized to tba-1 mRNA levels, and enrichment was expressed as fold change compared with the levels in total RNA. The graph represents mean and SE of 4–10 replicates. Virginie Freytag et al. J. Neurosci. 2017;37:6661-6672 ©2017 by Society for Neuroscience