l-2HG is elevated in RCC tumors and cell lines.

Slides:

Advertisements

Similar presentations

Actin PEA Hey KOC-7c OVCA420 OVCA432 OVTOKO RMG-1 SKOV3.ip1 Supplementary Fig. S1. Expression level of endogenous PEA-15 in ovarian cancer cell.

Advertisements

Supplemental data 2. Breast cancer primary tumor, metastasis and xenograft Total copy number gain (green), loss (red) and unchanged (black) for primary.

Glioma-derived soluble factor(s) enhance IL-10 signaling.

© 2016 S. Karger AG, Basel. All rights reserved Metabolomics of Chronic Kidney Disease Progression: A Case-Control Analysis in the Chronic Renal Insufficiency.

Homozygous deletions within chromosome 9q23.

B-cell precursors in the spleen of Eμ-Myc mice are reduced by mTORC1 inhibition. B-cell precursors in the spleen of Eμ-Myc mice are reduced by mTORC1 inhibition.

Altered cell migration factors and increased intestinal tumorigenesis in APC1638N/+ mice. Altered cell migration factors and increased intestinal tumorigenesis.

Development of preclinical models for the guidance of precision medicine. Development of preclinical models for the guidance of precision medicine. A,

PD-1 and LAG-3 expression in MSI and MSS colorectal cancer specimens.

Attenuated MDSC-suppressive activity and metastasis development in IDO-deficient mice is rescued by IL-6. Attenuated MDSC-suppressive activity and metastasis.

Geographic distribution in situ of MSI and MSS colorectal cancer–infiltrating lymphocytes. Geographic distribution in situ of MSI and MSS colorectal cancer–infiltrating.

The STM1787 promoter in Salmonella is rapidly activated in vivo by the tumor microenvironment. The STM1787 promoter in Salmonella is rapidly activated.

Expression of CD36 and psap in a TMA of human ovarian cancer patients

Bortezomib sensitivity correlates with basal NF-κB activity in KP lung adenocarcinoma cell lines. Bortezomib sensitivity correlates with basal NF-κB activity.

CHK1 downregulation upon ERG overexpression.

Epigenetic regulation of miR-193b in liposarcomagenesis.

Microarray analysis of phospho-Twist1–responsive genes.

Volume 18, Issue 1, Pages (January 2017)

PD-1 expression on HCC-infiltrating B cells and its clinical significance. PD-1 expression on HCC-infiltrating B cells and its clinical significance. A–H,

Mohamed A. Saleh et al. BTS 2016;1:

(A) Genotyping of the Lats2 and Cre alleles in the PyMT-derived cell lines (see primers location in Fig S2A). (A) Genotyping of the Lats2 and Cre alleles.

Core promoter methylation in mediators of adipogenesis.

qRT-PCR validation of differential expression.

Fig. 3 CSF1 is expressed in human melanoma.

Th1 and CTL-based immune signature and elevated checkpoint expression in MSI colorectal cancer. Th1 and CTL-based immune signature and elevated checkpoint.

Biochemical parameters of 143B cells.

Mineral content analysis by SEM-EDX.

Fig. 2. DDR1 over-expression enhances collagen fibril reorganization

SIRT1 is downregulated in human gastric cancer (GC).

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. JAK3A572V mutation causes constitutive JAK3 activity.

(A) Western blot probing nuclear extract from wild-type (wt) and the newly generated ACF1 mutant (AcfC) embryos (0–16 h). (A) Western blot probing nuclear.

Metformin and rapamycin decreased tumor weights in obese prediabetic mice. Metformin and rapamycin decreased tumor weights in obese prediabetic mice. A:

Abnormal cell cycle progression of Smo-deficient retinal progenitors.

BACHD mice demonstrate decreases in synaptic activity of medium-sized spiny neurons at 6 months. BACHD mice demonstrate decreases in synaptic activity.

TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor tissues. TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor.

Subcutaneous tumor growth was significantly increased in Ogt-Tg/+ mice

Socially isolated SV40 Tag mice develop significantly larger mammary gland tumors. Socially isolated SV40 Tag mice develop significantly larger mammary.

FAK overexpression in invasive human colon cancer and colon dysplasia.

LINC00092 binds with PFKFB2 to promote ovarian cancer metastasis.

A, scatterplots of qMSP analysis of candidate genes promoters in the prevalence screen cohort, which consisted of 55 HNSCC tumor tissue samples and 37.

AXL is not expressed in human prostate tumors.

EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo. EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo.

IDO deficiency delays the development of pulmonary metastases.

Microarray analysis of global gene expression profiles of PC-3 and derivative cell lines. Microarray analysis of global gene expression profiles of PC-3.

Ketogenic diets combined with fractionated radiation treatment results in decreased immunoreactive PCNA in tumor tissue. Ketogenic diets combined with.

Representative photograph of somatic mutations detected by PCR-SSCP.

Establishment of MajSAT RNA–expressing mice.

Immunohistochemical staining for FOXO3a of breast cancer tumor tissues

Reduced klotho expression in pancreatic cancer.

Activation status of CD8+ T cells.

Comparison of in vivo activity of 4D5scFvZZ and 4D5scFv.

IDO deficiency is associated with attenuated induction of IL-6 during 4T1 tumor metastasis. IDO deficiency is associated with attenuated induction of IL-6.

Specific reaction with MIB1, M30, and CD31 monoclonal antibodies in primary human cervical carcinoma before (A, C, and E) and after (B, D, and F) Tam treatment.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus tissues after stimulation in vivo with poly(I:C). Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus.

Validation of NAA's cancer specificity in lung cancer.

Long-range structural order control of SS-annealed cylinder patterns

Expression of chemokine receptors in A-498 cell line.

Leukocyte populations in normal and neoplastic breast tissues.

One potential implementation for the use of outlier kinase profiling and targeting for clinical management of pancreatic cancer in a precision medicine.

Heterozygous Pik3caH1047R oncogenic mutation causes invasive prostate cancer in mice that does not phenocopy Pten deletion. Heterozygous Pik3caH1047R oncogenic.

Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated breast cancer cells. Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated.

Frequency of CD4+CD25+FoxP3+ and CD4+CD25+CD127low T cells in unactivated PBMCs from healthy donors. Frequency of CD4+CD25+FoxP3+ and CD4+CD25+CD127low.

BRCA1 sequence variants classified as pathogenic do not restore HR

CD36 expression is coordinately regulated in multiple cellular compartments. CD36 expression is coordinately regulated in multiple cellular compartments.

CD36 expression in tissue adjacent and distal to the tumor.

Mutant TERT promoter displays active histone marks and distinct long-range interactions: A, cell lines that were used in the study with their origin and.

BIM expression predicts the response of patients with EGFR-mutant lung cancers. BIM expression predicts the response of patients with EGFR-mutant lung.

KDM4A levels affect the distribution of translation initiation factors

Treatment with octyl-D-2-HG and octyl-L-2-HG reduces MIR148A expression. Treatment with octyl-D-2-HG and octyl-L-2-HG reduces MIR148A expression. A, Treatment.

Presentation transcript:

l-2HG is elevated in RCC tumors and cell lines. l-2HG is elevated in RCC tumors and cell lines. Human kidney samples were obtained by surgical resection and metabolites were extracted for metabolite profiling analysis by GC/MS analysis. A, 2HG was significantly increased in primary tumor (tumor) compared with adjacent benign kidney tissue (normal). Inset graph is presented with smaller scale. B, high-2HG and low-2HG tumors were analyzed by tandem MS to resolve the enantiomeric distribution of 2HG within these tumors. C, relative ratio of d-2HG and l-2HG against total 2HG in high-2HG RCC samples. Error bars, SEM. *, P < 0.05. D, l-2HG levels were measured in another cohort of samples from a separate biorepository. E, enantiomeric resolution of 2HG in a panel of nontransformed and transformed lines of renal origin. WT, wild-type. Eun-Hee Shim et al. Cancer Discovery 2014;4:1290-1298 ©2014 by American Association for Cancer Research