Fig. 1 Synthesis and characterization of Dox-CBD-SA.

Slides:

Advertisements

Similar presentations

Fig. 4 3D reconfiguration of liquid metals for electronics.

Advertisements

Fig. 5 MicroLED array with 3D liquid metal interconnects.

Fig. 3 Cytosolic delivery of fluorescent proteins.

Aggregation kinetics of Aβ16–22 are unaffected by the presence of Aβ40

Fig. 3 The electrical contact of direct-printed and reconfigured liquid metals. The electrical contact of direct-printed and reconfigured liquid metals.

Fig. 2 Global production, use, and fate of polymer resins, synthetic fibers, and additives (1950 to 2015; in million metric tons). Global production, use,

Vibrational spectra of medieval human bones (Leopoli-Cencelle, Italy)

Fig. 1 Pump-probe signatures of vermilion (red HgS), black HgS, and metallic Hg. Pump-probe signatures of vermilion (red HgS), black HgS, and metallic.

Fig. 5 Thermal conductivity of n-type ZrCoBi-based half-Heuslers.

Fig. 6 Covalent inhibition of tuberculosis target MptpB.

Fig. 1 Examples of experimental stimuli and behavioral performance.

Fig. 1 Wireless, battery-free neural cuff for programmable pharmacology and optogenetics. Wireless, battery-free neural cuff for programmable pharmacology.

Fig. 4 Resynthesized complex boronic acid derivatives based on different scaffolds on a millimole scale and corresponding yields. Resynthesized complex.

HT synthesis of boronic acids using the building block approach

Fig. 1 Mean and median RCR (Relative Citation Ratio) of Roadmap Epigenomics Program research articles for each year. Mean and median RCR (Relative Citation.

Fig. 3 Glucose- and structure-dependent insulin release.

Fig. 1 Product lifetime distributions for the eight industrial use sectors plotted as log-normal probability distribution functions (PDF). Product lifetime.

Fig. 5 Structural photocycle for DmCry.

Fig. 3 FcRL1 is passively recruited into B cell immunological synapses upon BCR engagement in primary spleen B cells. FcRL1 is passively recruited into.

Characteristics of the topology-optimized actuator and long-term tests

Fig. 6 SNP rs is a functional SNP in 22Rv1 cells.

Fig. 3 Imaging DA release in the presence of DA receptor agonists and antagonists. Imaging DA release in the presence of DA receptor agonists and antagonists.

Fig. 1 Distribution of total and fake news shares.

Fig. 2 2D QWs of different propagation lengths.

Fig. 4 Organophosphorus compounds detected in the room temperature residues irradiated ices by multidimensional gas chromatography. Organophosphorus compounds.

Fig. 2 Hydrogel stress relaxation regulates cell cycle progression from the G1 phase to the S phase. Hydrogel stress relaxation regulates cell cycle progression.

Electrochemical characterization of pV4D4-coated silicon electrodes

Fig. 1 Experimental setup.

Fig. 1 Schematic illustration and atomic-scale rendering of a silica AFM tip sliding up and down a single-layer graphene step edge on an atomically flat.

Fig. 4 EUV TG signal from Si.

Fig. 6 WPS imaging of different chemical components in living cells.

Fig. 5 Schematic phase diagrams of Ising spin systems and Mott transition systems. Schematic phase diagrams of Ising spin systems and Mott transition systems.

Fig. 4 OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with different pH. OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with.

Fig. 5 Topology structure and strategy for the bottom-up synthesis of structurally uniform carboncones[1,m]. Topology structure and strategy for the bottom-up.

Fig. 4 Evolution of fraction of sickled RBCs under hypoxia.

Fig. 1 In vitro calibration plots and pH maps using iopamidol in human blood serum. In vitro calibration plots and pH maps using iopamidol in human blood.

Fig. 1 Time course of growth parameters for acetate-grown cultures of M. acetivorans with and without supplements. Time course of growth parameters for.

Fig. 3 Production of protein and Fe(II) at the end of growth correlated with increasing concentrations of ferrihydrite in the media that contained 0.2.

Fig. 2 Schematic drawings of Göbekli Tepe skulls.

Fig. 2 NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ clean air policy. NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ.

Fig. 3 Prophylactic or therapeutic use of DECON protects from herpes infections in vitro. Prophylactic or therapeutic use of DECON protects from herpes.

Fig. 4 Dox-CBD-SA treatment shows reduced toxicity.

Fig. 4 Relationships between light and economic parameters.

Fig. 5 Comparison of the liquid products generated from photocatalytic CO2 reduction reactions (CO2RR) and CO reduction reactions (CORR) on two catalysts.

Fig. 1 Experimental strategy and elemental and morphological analysis of 3D/2D perovskite bilayer. Experimental strategy and elemental and morphological.

Fig. 2 NP characterization.

Fig. 1 Location of the Jirzankal Cemetery.

Fig. 4 CO2 emission changes triggered by the JJJ clean air policy.

Fig. 6 MSC encapsulation in vitro within PdBT cross-linked gels.

Fig. 3 Maximal energy intake.

Multiplexed four- and eight-channel devices for rapid processing

Fig. 3 Effects of ASO and eGLP1-ASO conjugates on gene expression and protein levels in vitro in cell lines and primary mouse islet cells. Effects of ASO.

Experimenter gender and replicability in science

Fig. 3 Heterologous TRPV1-mediated uptake of gentamicin is attenuated by extracellular [Ca2+]. Heterologous TRPV1-mediated uptake of gentamicin is attenuated.

Fig. 3 Electronic conductivity studies.

Fig. 1 Schematic depiction of a paradigm for rapid and guided discovery of materials through iterative combination of ML with HiTp experimentation. Schematic.

Fig. 5 Swelling, reaction kinetics, hydrolytic degradation, and cytocompatibility of PdBT cross-linked hydrogels. Swelling, reaction kinetics, hydrolytic.

Fig. 4 Single-particle contact angle measurements.

Fig. 2 Supraballs and films from binary SPs.

Fig. 1 Schematic structure of a fluorescently labeled eGLP1-conjugated MALAT1 ASO and internalization of fluorescent eGLP1 and eGLP1-MALAT1-ASO. Schematic.

Fig. 4 Behavior of resistance peak near density nm = 5.

Fig. 1 Design principle and SEM characterization of super-origami DNA nanostructures with n-tuples. Design principle and SEM characterization of super-origami.

Fig. 3 Characterization of the luminescence temporal time traces.

Fig. 4 Characterization and SERS spectra of tetrameric metamolecules.

Fig. 5 Cytosolic delivery of toxic proteins.

Fig. 3 Three small molecules inhibit the transport of CCR5 and do not affect transport of CCR1 or CXCR4. Three small molecules inhibit the transport of.

Fig. 3 Calculated electronic structure of ZrCoBi.

Fig. 5 CD19-tPSMA(N9del) CAR T cell numbers in mouse and human.

Fig. 3 4n hybrids undergo bipolar and tripolar mitosis.

Fig. 3 Temperature-dependent heat capacity of YbTM2Zn20.

Presentation transcript:

Fig. 1 Synthesis and characterization of Dox-CBD-SA. Synthesis and characterization of Dox-CBD-SA. (A) Schematic of CBD-SA mediated drug delivery. (B) Synthesis scheme of Dox-CBD-SA. (C) Affinities [dissociation constant (Kd) values are shown] of CBD-SA and SA against collagen type I and collagen type III were measured by enzyme-linked immunosorbent assay (ELISA). N.D., not determined due to low signal. Graphs with [concentrations] versus [signals] are shown in fig. S2. Two experimental replicates. (D) Dox conjugation ratio per protein is presented. Values were calculated on the basis of the results of bicinchoninic acid assay protein quantification assay (proteins) and absorbance at 495 nm (Dox) (mean ± SD of three experimental replicates). (E) Dox release kinetics from Dox-CBD-SA under three different pH conditions was evaluated by fluorescence (excitation at 495 nm, emission at 590 nm) (n = 3, mean ± SD; two experimental replicates). (F) MMTV-PyMT cells were seeded and incubated overnight. Dox, Dox-SA, or Dox-CBD-SA was added (red). Cells were also stained with LysoTracker (green). Scale bars, 20 μm. Representative pictures are presented. Two experimental replicates. (G and H) Cytotoxicity of Dox variants against MMTV-PyMT cells or MC38 cells in vitro (n = 6, mean ± SEM). Two experimental replicates. IC50, half maximal inhibitory concentration. Koichi Sasaki et al. Sci Adv 2019;5:eaaw6081 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).