Methylene Blue Staining: A New Technique for Identifying Intersegmental Planes in Anatomic Segmentectomy  Zheng Zhang, MD, Yongde Liao, MD, PhD, Bo Ai,

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Methylene Blue Staining: A New Technique for Identifying Intersegmental Planes in Anatomic Segmentectomy  Zheng Zhang, MD, Yongde Liao, MD, PhD, Bo Ai, MD, Changyu Liu, MD  The Annals of Thoracic Surgery  Volume 99, Issue 1, Pages 238-242 (January 2015) DOI: 10.1016/j.athoracsur.2014.07.071 Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Methylene blue was injected into the segmental bronchus, and pictures were captured every 5 min for comparison. After the injection of methylene blue, the anterior segment of the left upper lobe turned blue, while the adjacent segment remained normal. (A) Five minutes later. (B) Thirty minutes later. The Annals of Thoracic Surgery 2015 99, 238-242DOI: (10.1016/j.athoracsur.2014.07.071) Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 (A) The target segmental vein, artery, and bronchus were cut off, and methylene blue was injected into the target bronchus using an intravenous infusion needle under thoracoscopic guidance. (B) After injection of the methylene blue, the apical segment of the right lower lobe was stained blue and the basal segment remained unstained. Half the target segment was cut off using the linear stapling device. (C) During open anatomic segmentectomy, 20 mL of 0.1% methylene blue was slowly injected into the left lingular bronchus using the intravenous infusion needle. (D) Use of the linear stapling device to cut off the lung tissue along the stained boundary. The Annals of Thoracic Surgery 2015 99, 238-242DOI: (10.1016/j.athoracsur.2014.07.071) Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions