Effect of mutations of the SRE and RCE on the Akt activation of the Fra-1 promoter. Effect of mutations of the SRE and RCE on the Akt activation of the Fra-1 promoter. The pFra4 reporter plasmid was mutated in either the SRE (mSRE) or the two RCE elements (mRCE1 and mRCE2) individually (A) or together (B). The reporter constructs were co-transfected with the control Renilla luciferase plasmid and either constitutively active Akt3 or the pcDNA3.1 vector as indicated. Forty-eight hours after transfection, the cells were lysed and luciferase activities measured. Results shown are the means ± SE of three independent experiments where the amount of firefly luciferase activity was normalized to the amount of Renilla luciferase activity measured in each sample. Gunjan Tiwari et al. Mol Cancer Res 2003;1:475-484 ©2003 by American Association for Cancer Research