Chromatographic Techniques Day 1 9:00 Introduction 9:15 Lecture: Gel filtration 10:00 Coffee Break 10:15 Introduction practical work and AKTA System demo 11:00 Exp 1: Gel Filtration-Desalting mode 11:30 Lecture: Ion Exchange 12:30 Lunch 1:30 Discussion results Exp.1/ Introduction Exp. 2 2:00 Practical: Exp2: Ion exchange- Media/pH scouting 2:30 Lecture: Analytical Techniques 3:30 Coffee Break 4:00 Discussion of results Exp. 2/ Intro Exp. 3 4:30 Practical: Exp.3: Ion exchange- binding capacity 6:00 End of day 1

Chromatographic Techniques Day 2 8:30 Discussion of results Exp. 3/ Intro Exp. 4 8:45 Practical: Exp.4: IEC scouting for step 2 9:30 Lecture: Purification Strategies 10:15 Coffee break 10:30 Analysis: Discussions results Exp.4 11:00 Lecture: Hydrophobic interactions & Reversed phase chromatography 12:00 Lunch 1:00 Intro Exp. 5 1:15 Practical: Exp.5: HIC-scouting 2:00 Lecture: Affinity chromatography 2:45 Analysis: Discussions results Exp. 5/ Intro Exp.6 3:15 Practical: Exp.6: IEC/ HIC contd. (step 2) 3:30 Coffee break 3:45 Lecture: Optimization (general) 5:00 End of day

Chromatographic Techniques Day 3 8:30 Lecture: Optimization (different techniques 9:30 Discussion of results Exp. 6/ Intro Exp. 7 9:45 Practical: Exp. 7: Affinity chromatography 10:15 Coffee break 10:30 Lecture: Scale up and fine tuning 11:30 Discussion of results Exp. 7 12:00 Lunch 1:00 Lecture: Process hygiene 1:45 Lecture: Regulation: A brief introduction 2:15 Coffee Break 2:30 Lecture: Column Packing (Video) 3:00 Discussion of results and Q&A 4:00 End of Course