Triple sugar iron (TSI) slant

Triple sugar iron (TSI) slant Albert Tsai, MD, PhD

Triple sugar iron (TSI) slant 1 glucose 10 lactose 10 sucrose acid pH indicator yellow O2 pH indicator peptone alkaline red ferric ammonium citrate + H2S black Albert Tsai, MD, PhD

Triple sugar iron (TSI) slant ferments lactose/sucrose produces H2S ferments glucose A/A E. coli Klebsiella Enterobacter Yersinia Serratia A/A+H2S Proteus runs out of glucose, cannot use lactose / sucrose, uses peptone cannot ferment, uses peptone produces H2S K/K, K/NC Pseudomonas Acinetobacter Eikenella Moraxella K/A Shigella Morganella Providencia K/A+H2S Salmonella Edwardsiella Albert Tsai, MD, PhD

Triple sugar iron (TSI) slant ferments lactose/sucrose produces H2S ferments glucose A/A E. coli Klebsiella Enterobacter Yersinia Serratia A/A+H2S Proteus runs out of glucose, cannot use lactose / sucrose, uses peptone cannot ferment, uses peptone produces H2S K/K, K/NC Pseudomonas Acinetobacter Eikenella Moraxella K/A Shigella Morganella Providencia K/A+H2S Salmonella Edwardsiella Albert Tsai, MD, PhD

N,N-dimethyl-p-phenylenediamine Oxidase test cytochrome oxidase + + + 4 4 3 O2 3 H2O N,N-dimethyl-p-phenylenediamine α naphthol – + indophenol blue http://pubchem.ncbi.nlm.nih.gov and http://www.pc.maricopa.edu/Biology/rcotter/

– + Catalase test + catalase 2 H2O2 2 H2O O2 hydrogen peroxide water oxygen – + http://staff.um.edu.mt/amce1/

– + Indole test + + + tryptophanase tryptophan water indole pyruvate ammonium Kovac reagent para-dimethylaminobenzaldehyde, isoamyl alcohol, concentrated HCl – + red Erlich reagent p-dimethylaminocinnamaldehyde blue-green http://pubchem.ncbi.nlm.nih.gov and http://www.uoit.ca/teachinglearning/VirtualLab

Nitrate reductase test nitrite reductase NO3- NO2- NH3 or N2 nitrate nitrite ammonia nitrogen α napthylamine, sulfanilic acid α napthylamine, sulfanilic acid α napthylamine, sulfanilic acid no change red no change zinc ? + + – zinc NO2- no change nitrite α napthylamine, sulfanilic acid red http://www.mesacc.edu/~johnson/labtools/Dbiochem/nit.html

Ornithine decarboxylase test ornithine decarboxylase + ornithine putrescine carbon dioxide – + pH indicator purple (alkaline) glucose fermentation yellow (acid) pH indicator http://pubchem.ncbi.nlm.nih.gov and http://www.vetmed.wisc.edu/pbs/courses/

– + Urease test + + urease H2O 2 NH3 CO2 carbon dioxide urea water ammonia phenol red – + fuschia (alkaline) http://pubchem.ncbi.nlm.nih.gov and http://totallyfreeimages.com/122631/