Expression of ylxR-containing operon revealed by lacZ and gfp fusion analysis. Expression of ylxR-containing operon revealed by lacZ and gfp fusion analysis. (A) β-Galactosidase activities from samples taken hourly are shown in Miller units. Means from three independent experiments and standard deviations are shown. The x axis represents the growth time in hours relative to the end of vegetative growth (T0). (Left) Cells were grown in sporulation medium with (closed symbols) or without (open symbols) 2% glucose. Circles and squares indicate the wild-type (OAM741) and cshA disruption mutant (OAM742), respectively. (Right) OAM741 cells were grown in sporulation medium with 3% sodium succinate (closed triangles) or 1% glycerol (closed inverted triangles) or without additional carbon sources (open circles). (B) Strain OAM818 (PylxS-gfp) was grown in sporulation medium with or without 2% glucose. After 14 h, cells were sampled and processed. Microscopic observation is shown. PC, phase contrast; GFP, green fluorescent protein. A histogram of fluorescence intensities of the cells with glucose is shown alongside the panel. GFP fluorescence was visualized using the NIBA filter set (Olympus). Image processing and data analysis were performed using Adobe Photoshop CS5. Mitsuo Ogura, and Yu Kanesaki mSphere 2018; doi:10.1128/mSphere.00501-18