Fibroblasts cultured from venous ulcers display cellular characteristics of senescence Manuel V. Mendez, MD, Andrew Stanley, MD, Hee-Young Park, PhD,

Slides:

Advertisements

Similar presentations

Timing of carotid endarterectomy after acute stroke Joseph J. Plotrowski, MD, Victor M. Bernhard, MD, Jeffrey R. Rubin, MD, Kenneth E. McIntyre, MD, James.

Advertisements

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

S. Sulaiman Shoab, MD, FCPS, FRCS, J. H. Scurr, MS, FRCS, P. D

Graft smooth muscle cells specifically synthesize increased collagen

Clinical evaluation of recombinant human platelet – derived growth factor for the treatment of lower extremity diabetic ulcers David L. Steed, MD, the.

The proliferative capacity of neonatal skin fibroblasts is reduced after exposure to venous ulcer wound fluid: A potential mechanism for senescence in.

Daniel J. Lindner, M. D. , James M. Edwards, M. D. , Edward S

Martorell's hypertensive ischemic leg ulcers are secondary to an increase in the local vascular resistance Henry J. Duncan, B.M., B.S., Irwin B. Faris,

Axillary-to-carotid artery bypass grafting for symptomatic severe common carotid artery occlusive disease Joseph P. Archie, PhD, MD Journal of Vascular.

Impact of a critical pathway on postoperative length of stay and outcomes after infrainguinal bypass Andrew C. Stanley, MD, Maryann Barry, RN, Thayer.

Surgical management of infected abdominal aortic grafts: Review of a 25-year experience Patrick J. O'Hara, M.D., Norman R. Hertzer, M.D., Edwin G. Beven,

A new valvulotome for in situ bypass grafts

Nicholas P. Ziats, PhD, James M. Anderson, MD, PhD

Pressure elevation slows the fibroblast response to wound healing

The proliferative capacity of neonatal skin fibroblasts is reduced after exposure to venous ulcer wound fluid: A potential mechanism for senescence in.

Michael A. Golden, MD, Y. P. Tina Au, PhD, Richard D

Differences in morphology, growth rate, and protein synthesis between cultured arterial and venous endothelial cells Willis H. Wagner, MD, Renee M. Henderson,

Malcolm O. Perry, MD, Richard Kempczinski, MD

Healing rates and cost efficacy of outpatient compression treatment for leg ulcers associated with venous insufficiency William A. Marston, MD, Robert.

A prospective, randomized trial of Unna's boot versus Duoderm CGF hydroactive dressing plus compression in the management of venous leg ulcers Paul R.

Definitive diagnosis and definitive treatment in chronic venous disease: A concept whose time has come Robert L. Kistner, MD Journal of Vascular Surgery

Timing of carotid endarterectomy after acute stroke

Marc M. Sedwitz, M. D. , Richard J. Davies, M. D. , Harold T

Carotid artery replacement in conjunction with resection of squamous cell carcinoma of the neck: Preliminary results M.Kathleen Reilly, PhD, MD, Malcolm.

David A. Lipski, MD, RVT, Alexander D. Shepard, MD, RVT, Bruce D

Daniel J. Lindner, M. D. , James M. Edwards, M. D. , Edward S

Limited B-mode venous imaging versus complete color-flow duplex venous scanning for detection of proximal deep venous thrombosis Roberta Poppiti, BS,

Transforming growth factor-β1 increases lysyl oxidase enzyme activity and mRNA in rat aortic smooth muscle cells Charles J. Shanley, MD, Mehrnaz Gharaee-Kermani,

Samuel S. Ahn, MD, David E. Williams, BA, Dirk A

Nicotine and cotinine stimulate secretion of basic fibroblast growth factor and affect expression of matrix metalloproteinases in cultured human smooth.

The Journal of Vascular Surgery: 1982 to 1990

Distribution and quantification of venous reflux in lower extremity chronic venous stasis disease with duplex scanning Michael S. Weingarten, MD, FACS,

Vivian Gahtan, MD, Xiu-Jie Wang, MD, Masataka Ikeda, MD, Alliric I

Unusual venous thrombosis associated with protein C deficiency

Treatment of venous disease – The innovators

A. N. Nicolaides, MS, FRCS, M. K. Hussein, MD, FRCS, G. Szendro, MD, D

David L. Gillespie, MD, Paul R

Lower extremity ischemia in adults younger than forty years of age: A community-wide survey of premature atherosclerotic arterial disease Pavel J. Levy,

Current use of inferior vena cava filters

Calvin B. Ernst, MD Journal of Vascular Surgery

Ruptured abdominal aortic aneurysm: A population-based study

Mesenteric venous thrombosis

Mesenteric blood flow in patients with diabetic neuropathy

Cardiovascular surgery—The rocket and its stars: Presidential address

William B. Shue, BA †, Sharon C. Worosilo, MS, Anthony P

Composite sequential bypasses to the ankle and beyond for limb salvage

Research and research funding in vascular surgery

Regarding “Venous ulcers and the superficial venous system”

J.Dennis Baker, MD Journal of Vascular Surgery

A comparison between descending phlebography and duplex Doppler investigation in the evaluation of reflux in chronic venous insufficiency: A challenge.

Septic embolism complicating infective endocarditis

Jay B. Fisher, MD, Marc A. Granson, MD Journal of Vascular Surgery

Should patient age be a consideration in carotid endarterectomy?

Evaluation of a proposed standard reporting system for preoperative angiograms in infrainguinal bypass procedures: Angiographic correlates of measured.

Acute aortic occlusion – Factors that influence outcome

Monitoring vascular surgical performance

Toe pressure determination by audiophotoplethysmography

William E. Evans, M.D., James P. Hayes, M.S.

Carotid endarterectomy for nonhemispheric cerebral symptoms: Patient selection with ocular pneumoplethysmography Kenneth Ouriel, M.D., John J. Ricotta,

D. Emerick Szilagyi, MD—An appreciation

Presidential address: The second-generation vascular surgeon

George D. Lilly 1906–1988 Journal of Vascular Surgery

James A. DeWeese, MD Journal of Vascular Surgery

Jerry Goldstone, MD Journal of Vascular Surgery

Axillary-to-carotid artery bypass grafting for symptomatic severe common carotid artery occlusive disease Joseph P. Archie, PhD, MD Journal of Vascular.

Innominate artery trauma: A thirty-year experience

Popliteal entrapment syndrome: A report of tibial nerve entrapment

Cornelius A. Sullivan, MD, Michael J. Rohrer, MD, Bruce S. Cutler, MD

Presentation transcript:

Fibroblasts cultured from venous ulcers display cellular characteristics of senescence Manuel V. Mendez, MD, Andrew Stanley, MD, Hee-Young Park, PhD, Karen Shon, Tania Phillips, MD, James O. Menzoian, MD Journal of Vascular Surgery Volume 28, Issue 5, Pages 876-883 (November 1998) DOI: 10.1016/S0741-5214(98)70064-3 Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Correlation between percentage of SA-β-Gal positive cells and the ratio between normal fibroblasts (normal-fb) and fibroblasts cultured from venous ulcers (wound-fb) growth rates. Journal of Vascular Surgery 1998 28, 876-883DOI: (10.1016/S0741-5214(98)70064-3) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Cellular fibronectin protein extracted from 80% to 90% confluent third passage fibroblasts isolated from venous ulcers (wound-fb) and uninvolved skin (normal-fb) of the ipsilateral thigh of the same patients. Densitometry was performed on scans from immunoblot autoradiograph using image analysis software. Results are presented in the table. Journal of Vascular Surgery 1998 28, 876-883DOI: (10.1016/S0741-5214(98)70064-3) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Cellular fibronectin messenger RNA (mRNA) extracted from 80% to 90% confluent third passage fibroblasts isolated from venous ulcers (wound-fb) and uninvolved skin (normal-fb) of the ipsilateral thigh of the same patients. Ethidium bromide labeled 18S subunit of total RNA loaded into each lane was used to calculate the ratio between cellular fibronectin mRNA and total RNA for purposes of comparison. Densitometry was performed on scans from Northern blot autoradiograph and picture of ethidium bromide labeled gel using image analysis software. Results are presented in table. Journal of Vascular Surgery 1998 28, 876-883DOI: (10.1016/S0741-5214(98)70064-3) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions