HETE-PEs are increased in leukocytes and platelets from APS patients.

Slides:

Advertisements

Similar presentations

CsEN components reveal precisely timed cellular programs that characterize the dynamic changes of the peripheral immune system over the course of pregnancy.

Advertisements

Soft robotic device applied to the RV in a pressure overload model of RHF. Soft robotic device applied to the RV in a pressure overload model of RHF. (A.

FIP200 loss links to poor autophagy and high apoptosis in naïve T cells in tumor. FIP200 loss links to poor autophagy and high apoptosis in naïve T cells.

FIP200 deficiency alters mitochondria activation and ROS production in T cells. FIP200 deficiency alters mitochondria activation and ROS production in.

Comparison of predicted and measured forces and moments.

TPAD controller schematic and testing for WPC.

TPAD controller performance for three force components.

FIP200 maintains microRNA1198-5p expression via Ago2 in naïve T cells.

Cable-driven system diagram for structure matrix.

Three different types of transfer functions with a codomain of [0,1].

TPAD training protocol.

Self-sensing of actuator position.

Examples of AEGIS autonomous target selection.

Workspace comparison of Delta robots.

T2-weighted cross-sectional MR imaging of MSP swarms inside SD rats.

Soft robotic device applied to the left side in a coronary ligation HF model. Soft robotic device applied to the left side in a coronary ligation HF model.

FIP200 loss links to poor autophagy and high apoptosis in naïve T cells in tumor. FIP200 loss links to poor autophagy and high apoptosis in naïve T cells.

Human PBMC-derived MERS-CoV–specific T cells are multifunctional.

Group data during free walking between sessions 1 and 16.

Soft robotic VAD implementations, control schemes, and HF models.

Virus-specific T cell responses are detected in all MERS survivors.

Arp2/3-mediated formation of nuclear actin networks is essential for CD4+ T cell effector functions. Arp2/3-mediated formation of nuclear actin networks.

Platelets are required for hFcγRIIA-induced anaphylaxis.

AEGIS intelligent targeting compared with blind targeting.

VH usage of cross-reactive B cells induced by H5N1 or H7N9 vaccination

Human cells produce type I and III IFNs upon Af stimulation.

Comparison of repertoire distributions to baseline.

Genetic FIP200 deletion impairs autophagy induction and causes T cell apoptosis. Genetic FIP200 deletion impairs autophagy induction and causes T cell.

Tukey boxplots overlaid on data points from objective and subjective measures, displaying results from study 1. Tukey boxplots overlaid on data points.

TCRs bind to CD1b-PG complexes formed in cells.

Soft robotic VAD implementations, control schemes, and HF models.

Tactile features for prosthesis perception.

Dectin-1 is repressed in allergic individuals.

Evidence for platelet activation during human drug-induced anaphylaxis

Online verification using reachable occupancies.

Illustration of the addressable wireless folding concept.

FIP200 maintains microRNA1198-5p expression via Ago2 in naïve T cells.

Cell viability tests. Cell viability tests. SEM images of (A) MC3T3-E1 cells and (B) MSCs on days 1, 3, and 5 of culture. (C) Survival rates of MC3T3-E1.

T2-weighted cross-sectional MR imaging of MSP swarms inside SD rats.

Prosthesis system diagram.

High numbers of non–islet-specific CTLs attenuate effector functions of islet-specific CTLs. High numbers of non–islet-specific CTLs attenuate effector.

Soft robotic device applied to the left side in a coronary ligation HF model. Soft robotic device applied to the left side in a coronary ligation HF model.

Evaluation of multidonor class Ig sequences obtained from H5N1 and H7N9 vaccinated donors. Evaluation of multidonor class Ig sequences obtained from H5N1.

Microrobots with different cell-carrying capacities under different grid lengths (lg) and burr lengths (lb). Microrobots with different cell-carrying capacities.

Platelet-released serotonin contributes to hypothermia in mice undergoing HA-hIgG–dependent anaphylaxis. Platelet-released serotonin contributes to hypothermia.

Self-sensing of actuator position.

Brain-computer interfaces.

Fig. 1 Examples of experimental stimuli and behavioral performance.

Slc7a5 expression in preosteoclasts is reduced in ovariectomized mice.

Soft robotic device applied to the RV in a pressure overload model of RHF. Soft robotic device applied to the RV in a pressure overload model of RHF. (A.

Nuclear accumulation of NFATc1 increases in Slc7a5-deficient osteoclasts. Nuclear accumulation of NFATc1 increases in Slc7a5-deficient osteoclasts. BMMs.

HFcγRIIA-expressing platelets are sufficient to induce anaphylaxis in resistant mice. hFcγRIIA-expressing platelets are sufficient to induce anaphylaxis.

Drug response profiles and experimental validation.

CsEN components reveal precisely timed cellular programs that characterize the dynamic changes of the peripheral immune system over the course of pregnancy.

Comparison of predicted and measured forces and moments.

Tfr cells respond better to immunization with self-antigens than with foreign antigens. Tfr cells respond better to immunization with self-antigens than.

Sensitivity analysis of cellular responses to perturbation of the DNA damage response signaling in the presence of chemotherapies. Sensitivity analysis.

Fig. 3 Proteomic analysis and Western blot analysis of protein cargos of various EVs. Proteomic analysis and Western blot analysis of protein cargos of.

Results of a representative participant with multiple training sessions. Results of a representative participant with multiple training sessions. Average.

Fig. 5 Simultaneous absence of caspase-3 and -7 is required for significant decrease of caspase-8 and -9 activation in intrinsic apoptosis. Simultaneous.

AEGIS autonomous targeting process.

Examples of AEGIS autonomous target selection.

Human Tfr cells do not express CD25.

Fig. 4. Paclitaxel promotes the expression of invasive isoforms of MENA in the primary breast cancer microenvironment. Paclitaxel promotes the expression.

Pharmacological targeting of CDs promotes response to KRASG12C inhibition in vivo. Pharmacological targeting of CDs promotes response to KRASG12C inhibition.

Enzyme activity of PE-GA/Pt tuned by changing temperature or NIR light

UNC drives MYC protein loss.

Fig. 5. High burdens of AA signature mutations and predicted immunogenicity in Taiwan HCCs. High burdens of AA signature mutations and predicted immunogenicity.

Fig. 1 Isopropanol tolerance variation among E. faecium isolates.

Neutralizing activity of mAbs isolated from plasmablasts during acute ZIKV infection in DENV-experienced donors. Neutralizing activity of mAbs isolated.

Presentation transcript:

HETE-PEs are increased in leukocytes and platelets from APS patients. HETE-PEs are increased in leukocytes and platelets from APS patients. (A and B) 15- and 5-HETE-PEs are increased in leukocytes from antiphospholipid syndrome (APS) patients. Total leukocytes were isolated from healthy control (HC) and APS patients, left unstimulated (US) or stimulated at 4 × 106 cells/ml with 10 μM A23187 for 30 min at 37°C, and then the lipids were analyzed for HETE-PEs. HETE-PEs were quantified as individual molecular species and then combined. Data are means ± SEM of 34 (HC) or 17 (APS) subjects. (C) 12-HETE-PEs are increased in platelets from APS patients. Washed platelets from HC and APS patients were isolated and left unstimulated or stimulated with thrombin (0.2 U/ml) for 30 min at 37°C before the amounts of 12-HETE-PE were determined. Data are means ± SEM of 18 (HC) or 12 (APS) subjects. (D) Platelets from APS patients that spontaneously aggregated contain substantial amounts of 12-HETE-PEs. During the final washing step, a number of APS platelet isolates spontaneously aggregated (n = 7 patients). Lipids were extracted and analyzed for 12-HETE-PEs. (E) Urinary 11-thromboxane B2 (TXB2) is increased in APS patients. Urine from HC and APS patients was analyzed by gas chromatography–mass spectrometry (MS) to determine the amounts of TXB2. Data are means ± SEM of 32 (HC) or 9 (APS) subjects. ng/mg creatinine (Cr). (F and G) 15- and 5-HETE-PEs are basally increased in concentration in platelets from APS patients. Platelets were prepared as described in (C) and then were analyzed to determine the amounts of the indicated HETE-PEs. Data are means ± SEM of 18 (HC) or 12 (APS) subjects. (H) 12-HETE-PEs are basally increased in abundance in leukocytes from APS patients. Total leukocytes were isolated and left unstimulated or stimulated at 4 × 106 cells/ml with 10 μM A23187 for 30 min at 37°C and then analyzed for 12-HETE-PEs. Data are means ± SEM of 34 (HC) or 18 (APS) subjects. ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05 by Mann-Whitney U test. Sarah N. Lauder et al., Sci. Signal. 2017;10:eaan2787 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works