Coexpression of Crf and Tac2 (A-P -1.5).

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Coexpression of Crf and Tac2 (A-P -1.5). Coexpression of Crf and Tac2 (A-P -1.5). A, Map of area examined B–E. B, DAPI stain (gray) of area examined. C, Crf expression (green) is found strongly in the CeL and CeM. D, Tac2 expression (red) is found strongly in the CeL and CeM. E. Overlay of B-D reveals strong overlap in expression of Crf and Tac2 in CeL but not CeM. Scale Bar indicates 50 µm. F, Quantification of single expressing cells and coexpressing Crf and Tac2 cells in CeC, CeL, and CeM. Bars represent the mean number of (co)expressing cells in each subcompartment. Data presented as mean ± SEM where *p < 0.05 difference between single and double-labeled populations (Mann–Whitney U test). Kenneth M. McCullough et al. eNeuro 2018;5:ENEURO.0010-18.2018 ©2018 by Society for Neuroscience