Dot-blot epitope mapping of Aβ1–42 conformers bound by TTR and IC50 values of binding. Dot-blot epitope mapping of Aβ1–42 conformers bound by TTR and IC50.

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Dot-blot epitope mapping of Aβ1–42 conformers bound by TTR and IC50 values of binding. Dot-blot epitope mapping of Aβ1–42 conformers bound by TTR and IC50 values of binding. A, TTR-bound Aβ1–42 conformers at the hydrophobic central region was demonstrated by epitope mapping dot blot. Aβ conformers (25 μm, 2 μl) were spotted on nitrocellulose membrane and incubated with antibody specific to different regions (N terminal Aβ3–10; middle, Aβ18–22, Aβ16–21; C terminal, Aβ30–35, Aβ35–39). The second spot on each strip is spotted with oligomers that were A11 (oligomer-specific) antibody positive, whereas the third spot is not. B, Quantification of binding (IC50) of biotinylated TTRs to Aβ1–42 conformers immobilized on Nunc Maxisorb plates. Xinyi Li et al. J. Neurosci. 2013;33:19423-19433 ©2013 by Society for Neuroscience