Domain analysis of GmPHD6 binding to LHP1, DNA element, and histone H3

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Domain analysis of GmPHD6 binding to LHP1, DNA element, and histone H3. Domain analysis of GmPHD6 binding to LHP1, DNA element, and histone H3. A, Western-blotting result of the GST pull-down assay. Domain arrangement of GmPHD6. The MBP-LHP1 fusion protein was pulled down by GST-G6P or GST-G6VP. The arrows pointed to the expressed GST or its fusion proteins with various domains of GmPHD6. B, EMSA assay of GmPHD6, G6N, and G6NM (V34M, E35V). 4× G-rich box (GTGGAG) elements were used in the EMSA assay. C, Western-blotting result of the peptide pull-down assay. GST-GmPHD6 or MBP-LHP1 fusion protein was pulled down by the biotin-conjugated histone H3 peptide. D, The N-terminal domain of GmPHD6 interacted with a low-methylated histone H3 peptide in a peptide pull-down assay. N: N-terminal domain, amino acids 1 to 115; V: Variable domain, amino acids 116 to 191; PHD finger: amino acids 192 to 248. Wei Wei et al. Plant Physiol. 2017;175:1304-1320 ©2017 by American Society of Plant Biologists